Kawahara Takeshi, Otani Hajime
Integrated Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, Japan.
Biosci Biotechnol Biochem. 2006 Feb;70(2):411-7. doi: 10.1271/bbb.70.411.
We investigated the effect of lactic acid bacteria (LAB) isolated from eight samples of commercially available Nozawana-zuke, a traditional Japanese pickle, on cytokine expression by mouse spleen cell cultures. The 12 isolated strains of LAB (Nz1-Nz12), which were identified as genus Lactobacillus or Leuconostoc by the API50CHL test, enhanced the expression of interferon (IFN)-gamma with 6 h of culture. Ten of these 12 LAB, particularly Nz8, enhanced interleukin (IL)-12 p40 expression. The actinase E- and Benzonase-treated or untreated cell wall fraction of Nz8 enhanced both IFN-gamma and IL-12 p40 expression, while the cell plasma fraction had little effect. In the presence of anti-toll like receptor 4 antibody, the effect of the cell wall fraction of Nz8 was significantly abrogated. These results suggest that some LAB from Nozawana-zuke have a T helper 1-type immunoenhancing effect.
我们研究了从八个市售日本传统腌菜野泽渍样本中分离出的乳酸菌（LAB）对小鼠脾细胞培养物中细胞因子表达的影响。通过API50CHL测试鉴定为乳酸杆菌属或明串珠菌属的12株分离LAB菌株（Nz1-Nz12），在培养6小时后增强了干扰素（IFN）-γ的表达。这12株LAB中的10株，特别是Nz8，增强了白细胞介素（IL）-12 p40的表达。Nz8经肌动蛋白酶E和核酸酶处理或未处理的细胞壁组分增强了IFN-γ和IL-12 p40的表达，而细胞质组分的影响很小。在存在抗Toll样受体4抗体的情况下，Nz8细胞壁组分的作用被显著消除。这些结果表明，来自野泽渍的一些LAB具有辅助性T细胞1型免疫增强作用。
