柘树中异戊烯基黄酮类化合物的选择性ABTS自由基清除活性
Selective ABTS radical-scavenging activity of prenylated flavonoids from Cudrania tricuspidata.
作者信息
Lee Byong Won, Lee Jin Hwan, Gal Sang Wan, Moon Yea Hwang, Park Ki Hun
机构信息
Division of Applied Life Science (BK21 program), Department of Agricultural Chemistry, Institute of Agriculture & Life Sciences, Gyeongsang National University, Japan.
出版信息
Biosci Biotechnol Biochem. 2006 Feb;70(2):427-32. doi: 10.1271/bbb.70.427.
The antioxidative properties of five prenylated flavonoids, including new flavanone (2), from the root bark of Cudrania tricuspidata were examined against the ABTS, DPPH, and hydroxyl radicals. In most of the assays to determine their antioxidative properties, the ABTS activity was strongly correlated with DPPH because both methods are responsible for the same chemical property of hydrogen- or electron-donation to the antioxidant. On the other hand, the prenylated flavonoids (1-5) acted differently with both methods; namely, all the prenylated flavonoids strongly scavenged the ABTS radical (IC(50) < 10 microM), while they were inactive against the DPPH radical (IC(50) > 300 microM). Even though isolated 5,7,2',4',-tetrahydroxy-6,5'-diprenylflavanone (3) showed weak reducing power (746 mV) by cyclic voltammetry when compared to quercetin (394 mV), both had similar ABTS activity (IC(50) < 5 microM).
对柘树根皮中的5种异戊烯基黄酮类化合物（包括新黄烷酮（2））针对ABTS、DPPH和羟基自由基的抗氧化性能进行了检测。在大多数测定其抗氧化性能的试验中，ABTS活性与DPPH密切相关，因为这两种方法针对的是抗氧化剂相同的供氢或供电子化学性质。另一方面，异戊烯基黄酮类化合物（1 - 5）在这两种方法中的表现不同；也就是说，所有异戊烯基黄酮类化合物都能强烈清除ABTS自由基（IC(50) < 10 microM），而它们对DPPH自由基无活性（IC(50) > 300 microM）。尽管通过循环伏安法测定，分离得到的5,7,2',4', - 四羟基 - 6,5'-二异戊烯基黄烷酮（3）与槲皮素（394 mV）相比显示出较弱还原能力（746 mV），但二者具有相似的ABTS活性（IC(50) < 5 microM）。
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