Mendoza Montero J, Navarro Marí J M, Rojas González A, Plata Rosales J C, de la Rosa Fraile M
Servicio de Microbiología, Ciudad Sanitaria Virgen de las Nieves, Granada.
Enferm Infecc Microbiol Clin. 1991 Feb;9(2):100-1.
Detection of cytomegalovirus has been carried out in 178 clinical samples in order to compare the effectiveness of two distinct commercial immunofluorescence reagents: anti-CMV early nuclear protein (Du Pont) and CMV culture identification (Syva Micro-Trak). In 61 of the samples we observed positive reaction to either of the two procedures (Shell-vial and, or, cellular conventional culture). Sensitivity was 0.92 for Du Pont reagent, 0.79 for Syva reagent, and 0.93 for the conventional cellular culture. In the overall samples we observed a higher incidence of fluorescent focus using Du Pont reagent that using that of Syva.
为比较两种不同的商业免疫荧光试剂——抗巨细胞病毒早期核蛋白试剂（杜邦公司）和巨细胞病毒培养鉴定试剂（赛瓦公司的Micro-Trak）的有效性，对178份临床样本进行了巨细胞病毒检测。在61份样本中，我们观察到两种检测方法（空斑试验和/或细胞常规培养）中的任一种出现阳性反应。杜邦试剂的灵敏度为0.92，赛瓦试剂的灵敏度为0.79，细胞常规培养的灵敏度为0.93。在所有样本中，我们观察到使用杜邦试剂时荧光灶的发生率高于使用赛瓦试剂时的发生率。
