Pulido M A, Escribano E, López P, Ayats J, Pérez J L, Martín R
Servicio de Microbiología, Hospital de Bellvitge-Prínceps d'Espanya, Universidad de Barcelona.
Enferm Infecc Microbiol Clin. 1991 Nov;9(9):564-6.
We performed an evaluation of early cytomegalovirus antigen detection (Shell-vial method) for rapid diagnosis of cytomegalovirus infection in patients at risk. A comparative, parallel, prospective study against conventional cell culture in 913 clinical specimens was carried out. Shell-vial technique detected an early cytomegalovirus antigen in culture using an indirect immunofluorescent method with monoclonal antibodies. Cytomegalovirus was detected in 210 specimens (23.0%). In 24 instances (11.4%), cytomegalovirus was only detected by culture, in contrast with 54 specimens (25.7%) that were only positive in Shell-vial (p less than 0.001; McNemar test). Mean detection time for cell culture was 9.2 days, and 16-24 h for the Shell-vial technique. This is a rapid, sensitive, specific and simple to perform method for diagnosis of cytomegalovirus infection. Nevertheless, the predictivity of cytomegalovirus disease was not improved by this technique.
我们对早期巨细胞病毒抗原检测（空斑小瓶法）进行了评估，以快速诊断高危患者的巨细胞病毒感染。针对913份临床标本，开展了一项与传统细胞培养法进行对比的平行前瞻性研究。空斑小瓶技术使用单克隆抗体间接免疫荧光法在培养物中检测早期巨细胞病毒抗原。在210份标本（23.0%）中检测到巨细胞病毒。在24例（11.4%）中，仅通过培养检测到巨细胞病毒，相比之下，有54份标本（25.7%）仅在空斑小瓶检测中呈阳性（p<0.001；McNemar检验）。细胞培养的平均检测时间为9.2天，空斑小瓶技术的检测时间为16 - 24小时。这是一种用于诊断巨细胞病毒感染的快速、灵敏、特异且操作简便的方法。然而，该技术并未提高巨细胞病毒疾病的预测性。
