Lipson S M, Falk L H, Lee S H
Jane and Dayton Brown and Dayton T. Brown, Jr., Virology Laboratory, Department of Medicine, North Shore University Hospital-Cornell University Medical College, Manhasset, NY 11030, USA.
Arch Pathol Lab Med. 1996 Jan;120(1):53-6.
To investigate the rate of Cytomegalovirus (CMV) detection in peripheral blood by shell vial assay-indirect immunofluorescent assay technology using two leukocyte inocula concentrations and different inoculum volumes containing equivalent cell concentrations.
Leukocyte inocula concentrations of 2 x 10(5) and 4 x 10(5) cells per 0.2 mL were assayed for the presence of CMV by shell vial assay-indirect immunofluorescent assay. The effect of different inoculum volumes (0.2 and 0.4 mL) containing an equivalent cell concentration of 4 x 10(5) was evaluated as well. The data were compared to conventional MRC-5 tube cultures, including blind passage.
Ninety-five patients (101 specimens) were tested sequentially. The test population consisted primarily of patients suffering from the acquired immunodeficiency syndrome.
The diagnostic virology laboratory, acquired immunodeficiency syndrome clinics, and hospital wards.
Among the 101 specimens tested by shell vial assay-indirect immunofluorescent assay, the rate of CMV sensitivity increased by 36% using the higher leukocyte inoculum concentration of 4 x 10(5) cells per 0.2 mL (P = .002; Cochran's Q test). No significant difference in CMV yield was identified using equivalent cell concentrations with inocula volumes of 0.2 or 0.4 mL. The CMV sensitivity rate using the higher leukocyte inoculum surpassed that obtained by conventional tube culture-blind passage.
These data denote the importance of leukocyte concentration on the rate of CMV detection in peripheral blood by the shell vial assay-indirect immunofluorescent assay. The data also point out the need to establish a standardized blood preparation protocol to achieve optimal clinical relevance of this widely used laboratory test.
采用空斑试验-间接免疫荧光检测技术，使用两种白细胞接种浓度以及含有等量细胞浓度的不同接种体积，研究外周血中巨细胞病毒（CMV）的检出率。
采用空斑试验-间接免疫荧光检测法，检测每0.2 mL中含2×10⁵和4×10⁵个细胞的白细胞接种浓度中CMV的存在情况。还评估了含有等量4×10⁵个细胞浓度的不同接种体积（0.2 mL和0.4 mL）的效果。将数据与传统的MRC-5管培养法（包括盲传）进行比较。
对95例患者（101份标本）进行了连续检测。受试人群主要为获得性免疫缺陷综合征患者。
诊断病毒学实验室、获得性免疫缺陷综合征诊所和医院病房。
在通过空斑试验-间接免疫荧光检测法检测的101份标本中，使用每0.2 mL中含4×10⁵个细胞的较高白细胞接种浓度时，CMV敏感性率提高了36%（P = 0.002； Cochr an Q检验）。使用等量细胞浓度且接种体积为0.2 mL或0.4 mL时，CMV产量无显著差异。使用较高白细胞接种浓度时的CMV敏感性率超过了传统管培养-盲传法获得的敏感性率。
这些数据表明白细胞浓度对外周血中CMV通过空斑试验-间接免疫荧光检测法的检出率具有重要意义。数据还指出需要建立标准化的血液制备方案，以实现这种广泛使用的实验室检测的最佳临床相关性。
