Postollec F, Norde W, de Vries J, Busscher H J, van der Mei H C
Department of Biomedical Engineering, University Medical Center Groningen, and University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
J Dent Res. 2006 Mar;85(3):231-4. doi: 10.1177/154405910608500305.
The temporo-spatial development of plaque is governed by adhesive interactions between different co-aggregating bacterial strains and species. Physico-chemically, these interactions are due to attractive Lifshitz-Van der Waals and acid-base forces, and occur despite electrostatic repulsion and with a critical influence of temperature. The forces between co-aggregating and non-co-aggregating pairs have never been measured, however. The aim here, thus, is to investigate, by atomic force microscopy, whether there is a difference in interactive forces between co-aggregating and non-co-aggregating bacterial pairs at 10 degrees C, 22 degrees C, and 40 degrees C. Actinomyces naeslundii 147 was immobilized on poly-L-lysine-coated tipless AFM cantilevers, while streptococci were immobilized on poly-L-lysine-coated glass surfaces. Upon approach, a repulsive force was measured, regardless of whether a co-aggregating or non-co-aggregating pair was involved. However, upon retraction, the co-aggregating pair exhibited larger adhesive forces and energies than did the non-co-aggregating pair. Adhesive interactions between the co-aggregating pair were smallest at 40 degrees C.
菌斑的时空发展受不同共聚集细菌菌株和种类之间的黏附相互作用支配。从物理化学角度来看，这些相互作用归因于吸引性的 Lifshitz -范德华力和酸碱力，尽管存在静电排斥且受温度的关键影响，但仍会发生。然而，共聚集和非共聚集细菌对之间的力从未被测量过。因此，这里的目的是通过原子力显微镜研究在10摄氏度、22摄氏度和40摄氏度下，共聚集和非共聚集细菌对之间的相互作用力是否存在差异。内氏放线菌147固定在聚-L-赖氨酸包被的无尖原子力显微镜悬臂上，而链球菌固定在聚-L-赖氨酸包被的玻璃表面。在靠近时，无论涉及的是共聚集对还是非共聚集对，都测量到了排斥力。然而，在回缩时，共聚集对比非共聚集对表现出更大的黏附力和能量。共聚集对之间的黏附相互作用在40摄氏度时最小。
