Tsarev S A, Emerson S U, Balayan M S, Ticehurst J, Purcell R H
Hepatitis Viruses Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
J Gen Virol. 1991 Jul;72 ( Pt 7):1677-83. doi: 10.1099/0022-1317-72-7-1677.
Fragments of cDNA representing greater than 99% of the entire genome of wild-type hepatitis A virus (HAV) strain AGM-27, isolated from an African green monkey, were obtained by the polymerase chain reaction and sequenced. Comparison with other HAV isolates revealed differences in the predicted amino acid sequence in functionally critical parts of the genome. Comparison of the biological properties of AGM-27 with those of human wild-type and cell culture-adapted HM-175 strains revealed that AGM-27 grew in cell culture significantly better than did wild-type HM-175, but not as well as cell culture-adapted HM-175. AGM-27 and cell culture-adapted HM-175 were distinguishable by their differential growth in CV-1, FRhK-4 and primary AGMK cells.
从一只非洲绿猴分离出的野生型甲型肝炎病毒（HAV）AGM - 27株，其大于99%的全基因组cDNA片段通过聚合酶链反应获得并测序。与其他HAV分离株的比较显示，在基因组功能关键部分的预测氨基酸序列存在差异。将AGM - 27的生物学特性与人类野生型及细胞培养适应型HM - 175株的生物学特性进行比较，结果显示AGM - 27在细胞培养中的生长明显优于野生型HM - 175，但不如细胞培养适应型HM - 175。AGM - 27和细胞培养适应型HM - 175在CV - 1、FRhK - 4和原代AGMK细胞中的生长差异可将它们区分开来。
