人类和猿猴甲型肝炎病毒中编码RNA聚合酶的基因组片段的变异

Variations in genome fragments coding for RNA polymerase in human and simian hepatitis A viruses.

作者信息

Balayan M S, Andjaparidze A G, Tsarev S A, Sverdlov E D, Chizhikov V E, Blinov V M, Vasilenko S K

机构信息

Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, M.M. Shemyakin Institute of Bioorganic Chemistry, Moscow.

出版信息

FEBS Lett. 1989 Apr 24;247(2):425-8. doi: 10.1016/0014-5793(89)81384-5.

DOI:10.1016/0014-5793(89)81384-5

PMID:2541023

Abstract

The genome of hepatitis A virus (HAV) isolated from spontaneously infected African vervet monkey (Cercopithecus aethiops) has been cloned and partially sequenced. Comparison of genome fragments (1248 and 162 bp) from the 3D (RNA polymerase) region with the corresponding parts of human HAV genomes revealed a high degree of heterogeneity: there were altogether 257 nucleotide changes leading to 44 substitutions in predicted amino acid sequence, i.e. 89% amino acid identity. This divergence is considered to be significantly greater than genomic variations usually found among human HAV strains, where amino acid identity in the 3D region is over 98%.

摘要

从自然感染的非洲绿猴（长尾黑颚猴）中分离出的甲型肝炎病毒（HAV）基因组已被克隆并进行了部分测序。将来自3D（RNA聚合酶）区域的基因组片段（1248和162 bp）与人类HAV基因组的相应部分进行比较，发现存在高度异质性：总共有257个核苷酸变化，导致预测氨基酸序列中有44个替换，即氨基酸同一性为89%。这种差异被认为明显大于通常在人类HAV毒株中发现的基因组变异，在人类HAV毒株中，3D区域的氨基酸同一性超过98%。