血清中和试验与间接免疫荧光试验在检测牛传染性鼻气管炎病毒特异性抗体方面的相关性。
Correlation between the serum neutralization test and the indirect immunofluorescent test for the detection of specific antibodies to infectious bovine rhinotracheitis virus.
作者信息
Assaf R, Marsolais G, Marois P, Payment P
出版信息
Can J Comp Med. 1975 Apr;39(2):224-6.
Abstract
Comparative titrations show that immunofluorescence on infected rabbit kidney cells is relatively as sensitive as the neutralization test for the detection of antibodies to the infectious bovine rhinotracheitis virus. When calf testis cells infected with this virus are used for the detection of antibodies by the indirect immunofluorescent test nonspecific staining is high. This nonspecific reaction is absent when rabbit kidney cells are used.
摘要
比较滴定法表明，在检测牛传染性鼻气管炎病毒抗体时，感染兔肾细胞上的免疫荧光与中和试验的灵敏度相当。当用感染该病毒的犊牛睾丸细胞通过间接免疫荧光试验检测抗体时，非特异性染色较高。而使用兔肾细胞时则不存在这种非特异性反应。
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