Nakamura K, Ienaga K, Yokozawa T, Fujitsuka N, Oura H
Department of Natural Products Chemistry, Institute of Bio-Active Science (IBAS), Nippon Zoki Pharmaceutical Co. Ltd., Hyogo, Japan.
Nephron. 1991;58(1):42-6. doi: 10.1159/000186376.
The chemical oxidative conversion of creatinine (Cr) into methylguanidine (MG) has been followed by 1H-NMR and HPLC. By using active oxygen species generated by Fenton's reagent (Fe2+ and H2O2) or the similar reagent (Fe3+ and H2O2), creatol (CTL), a metabolite newly isolated from the urine of uremic patients, and creatones A and B were experimentally detected in the reaction mixture and implicated as successive intermediates in the pathway from CR to MG. An alternative oxidation of Cr to demethylcreatinine (glycocyamidine) was also observed. The importance of CTL in this in vitro oxidation mechanism is discussed.
通过1H-NMR和HPLC跟踪了肌酐（Cr）化学氧化转化为甲基胍（MG）的过程。利用芬顿试剂（Fe2+和H2O2）或类似试剂（Fe3+和H2O2）产生的活性氧物种，在反应混合物中通过实验检测到了肌醇（CTL）（一种新从尿毒症患者尿液中分离出的代谢产物）以及肌酮A和B，并认为它们是从CR到MG途径中的连续中间体。还观察到Cr向去甲基肌酐（胍基乙酸）的另一种氧化反应。讨论了CTL在这种体外氧化机制中的重要性。
