Langlais Carly L, Jones John M, Estep Ryan D, Wong Scott W
Vaccine and Gene Therapy Institute, Oregon Health & Science University, West Campus, 505 N.W. 185th Avenue, Beaverton, Oregon 97006, USA.
J Virol. 2006 Mar;80(6):3098-103. doi: 10.1128/JVI.80.6.3098-3103.2006.
A viral CD200 homologue (vCD200) encoded by open reading frame R15 of rhesus rhadinovirus (RRV), a gammaherpesvirus closely related to human herpesvirus 8 (HHV-8), is described here. RRV vCD200 shares 30% and 28% amino acid identity with human CD200 (huCD200) and HHV-8 vCD200, respectively. In vitro analysis indicated that an Fc fusion (vCD200-Fc) is expressed as a glycoprotein with a core molecular mass of 53 kDa. Utilizing monoclonal antibodies raised against vCD200-Fc, vCD200 expression was detected on the surfaces of and within supernatants from infected fibroblasts. Furthermore, in vitro assays demonstrated that vCD200-Fc treatment of monocyte-derived macrophages reduces tumor necrosis factor transcript and protein levels, implying that RRV encodes a functional vCD200.
本文描述了一种由恒河猴疱疹病毒（RRV）的开放阅读框R15编码的病毒CD200同源物（vCD200），RRV是一种与人类疱疹病毒8型（HHV-8）密切相关的γ疱疹病毒。RRV vCD200与人类CD200（huCD200）和HHV-8 vCD200的氨基酸同一性分别为30%和28%。体外分析表明，Fc融合蛋白（vCD200-Fc）表达为一种核心分子量为53 kDa的糖蛋白。利用针对vCD200-Fc产生的单克隆抗体，在感染的成纤维细胞表面和上清液中检测到了vCD200的表达。此外，体外试验表明，用vCD200-Fc处理单核细胞衍生的巨噬细胞可降低肿瘤坏死因子的转录水平和蛋白水平，这意味着RRV编码一种功能性vCD200。
