Doi S, Ohno H, Tatsumi E, Arita Y, Kamesaki H, Fukuhara S, Nishikori M, Miwa H, Kita K, Hatanaka M
First Department of Internal Medicine, Kyoto University, Japan.
Blood. 1987 Nov;70(5):1619-23.
A novel cell line, FL-18, was established from the pleural effusion of a patient with follicular small cleaved cell lymphoma. At the same time, an Epstein-Barr virus (EBV) nuclear antigen (EBNA)-positive cell line, FL-18-EB, was established from the EBV-infected culture of the same pleural effusion cells. Both cell lines had the same monoclonal surface immunoglobulin (IgG kappa), and they had the same karyotype as that of the fresh pleural effusion cells in which a reciprocal translocation between the long arm of chromosomes 14 and 18 [t(14;18)(q32;q21)] was detected. Gene rearrangement analysis of immunoglobulin heavy-chain gene (JH) and kappa light-chain gene (J kappa) showed the same rearranged configuration in the two cell lines; however, some morphological and phenotypic differences were found. The FL-18-EB cells, which were morphologically similar to common EBNA-positive lymphoblastoid cell lines of normal B cell origin at the initial phase of culture, were larger than the FL-18 cells and contained multinucleated giant cells. The FL-18 cells lacked cytoplasmic immunoglobulin and were positive for common acute lymphoblastic leukemia antigen (CALLA), whereas the FL-18-EB cells had cytoplasmic immunoglobulin and were negative for CALLA. Thus, the phenotype of FL-18-EB seems to be a result of a shift by EBV infection to a more mature stage in the B cell differentiation pathway than that of FL-18. The paired availability of EBV-free and EBV-infected cell lines of a neoplastic clone is unique and valuable in considering EBV infectibility of neoplastic B cells and resultant phenotypic changes.
一种新的细胞系FL-18是从一名滤泡性小裂细胞淋巴瘤患者的胸腔积液中建立的。同时，从同一胸腔积液细胞的EB病毒（EBV）感染培养物中建立了一种EBV核抗原（EBNA）阳性细胞系FL-18-EB。两种细胞系具有相同的单克隆表面免疫球蛋白（IgG κ），并且它们与新鲜胸腔积液细胞具有相同的核型，在新鲜胸腔积液细胞中检测到染色体14和18长臂之间的相互易位[t(14;18)(q32;q21)]。免疫球蛋白重链基因（JH）和κ轻链基因（Jκ）的基因重排分析显示，两种细胞系具有相同的重排构型；然而，发现了一些形态学和表型差异。FL-18-EB细胞在培养初期形态上与正常B细胞来源的常见EBNA阳性淋巴母细胞系相似，比FL-18细胞大，并且含有多核巨细胞。FL-18细胞缺乏细胞质免疫球蛋白，对常见急性淋巴细胞白血病抗原（CALLA）呈阳性，而FL-18-EB细胞具有细胞质免疫球蛋白，对CALLA呈阴性。因此，FL-18-EB的表型似乎是EBV感染导致B细胞分化途径比FL-18向更成熟阶段转变的结果。肿瘤克隆的无EBV和EBV感染细胞系的配对可用性在考虑肿瘤B细胞的EBV感染性和由此产生的表型变化方面是独特且有价值的。
