Andrade Sonia Gumes, Campos Rozália Figueira, Sobral Karina Souza Castro, Magalhães Juracy Barbosa, Guedes Ricardo S Pereira, Guerreiro Marcos Lázaro
Laboratório de Chagas Experimental, Autoimunidade e Imunologia Celular, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA.
Rev Soc Bras Med Trop. 2006 Jan-Feb;39(1):1-8. doi: 10.1590/s0037-86822006000100001. Epub 2006 Feb 23.
Reinfections with Trypanosoma cruzi in patients from endemic areas have been claimed to be an aggravation factor of cardiac manifestations in Chagas' disease. In the present study, the influence of triple infections with strains of different biodemes, on cardiac and skeletal muscle lesions was experimentally tested. Fifty eight mice chronically infected with the Colombian strain (Biodeme Type III) were successively reinfected as follows: 1st group--reinfected with 21 SF strain (Type II) followed by Y strain (Type I ); 2nd--group reinfections with Y strain followed by 21SF strain. Isoenzyme analysis of parasites from hemocultures obtained from triple infected mice, revealed the patterns of three distinct zymodemes in the same animal. Each Trypanosoma cruzi strain was reisolated after four passages in mice on either the 7th, 14th or 30th day after inoculation with the blood of triple infected mice. Histopathology results demonstrated a significant exacerbation of cardiac and skeletal muscle inflammatory lesions, confirmed by morphometric evaluation, in mice with triple infection. No aggravation of parasitism was detected. The possibility of an enhancement of cellular response in the triple infected mice is suggested.
来自流行地区的克氏锥虫再感染被认为是恰加斯病心脏表现的一个加重因素。在本研究中，对不同生物型菌株的三重感染对心脏和骨骼肌病变的影响进行了实验测试。58只慢性感染哥伦比亚菌株（生物型III型）的小鼠按以下方式相继再感染：第一组——先感染21SF菌株（II型），再感染Y菌株（I型）；第二组——先感染Y菌株，再感染21SF菌株。对三重感染小鼠血液培养物中的寄生虫进行同工酶分析，结果显示同一动物体内有三种不同酶型的模式。在接种三重感染小鼠血液后的第7、14或30天，每种克氏锥虫菌株在小鼠体内传代四次后均被重新分离出来。组织病理学结果表明，经形态计量学评估证实，三重感染小鼠的心脏和骨骼肌炎症病变明显加重。未检测到寄生虫感染加重的情况。提示三重感染小鼠存在细胞反应增强的可能性。
