Elia Antonia Concetta, Anastasi Valeria, Dörr Ambrosius Josef Martin
Dipartimento di Biologia Cellulare e Ambientale, Via Elce di Sotto, Università di Perugia, 06100 Perugia, PG, Italy.
Chemosphere. 2006 Sep;64(10):1633-41. doi: 10.1016/j.chemosphere.2006.01.035. Epub 2006 Feb 28.
This study was carried out in order to assess the effects of disinfectant-treatment on antioxidant response of Cyprinus carpio L. Therefore, enzymatic activities of glutathione S-transferases, glyoxalase I, glyoxalase II, glutathione peroxidases, glutathione reductase, catalase and total glutathione content of carp liver, exposed to surface water treated with three disinfectants for potabilization, sodium hypochlorite, chlorine dioxide and peracetic acid were investigated. Specimens of carp were exposed in four experimental tanks supplied with a continuous water flow from Lake Trasimeno (Italy), three of them treated with constant concentration of sodium hypochlorite, chlorine dioxide and peracetic acid, for 10 and 20 days, while the control tank was supplied with untreated lake water. Differences in biochemical parameters were observed in specimens following exposure to these disinfectants and mainly, chlorine compounds induced marked biochemical variations of carp liver, compared to those induced by peracetic acid treatment. Our results showed that antioxidant parameters of Cyprinus carpio could be used as biomarkers of oxidative stress when this species is exposed to disinfectants for water potabilization.
本研究旨在评估消毒剂处理对鲤鱼抗氧化反应的影响。因此，研究了暴露于用三种用于饮用水净化的消毒剂（次氯酸钠、二氧化氯和过氧乙酸）处理过的地表水的鲤鱼肝脏中谷胱甘肽S-转移酶、乙二醛酶I、乙二醛酶II、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、过氧化氢酶的酶活性以及总谷胱甘肽含量。鲤鱼样本暴露于四个实验水箱中，水箱持续供应来自意大利特拉西梅诺湖的水流，其中三个水箱用恒定浓度的次氯酸钠、二氧化氯和过氧乙酸处理10天和20天，而对照水箱供应未处理的湖水。在暴露于这些消毒剂后的样本中观察到生化参数的差异，主要是与过氧乙酸处理相比，含氯化合物引起鲤鱼肝脏明显的生化变化。我们的结果表明，当鲤鱼暴露于用于饮用水净化的消毒剂时，其抗氧化参数可作为氧化应激的生物标志物。
