投喂不同水平苏氨酸饲料的亚成年草鱼（Ctenopharyngodon idella）肠道和肝胰腺的生长、消化吸收能力及抗氧化状态
Growth, digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp Ctenopharyngodonidella fed graded levels of dietary threonine.
作者信息
Hong Yang, Jiang Weidan, Kuang Shengyao, Hu Kai, Tang Ling, Liu Yang, Jiang Jun, Zhang Yongan, Zhou Xiaoqiu, Feng Lin
机构信息
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan China.
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130 Sichuan China ; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130 Sichuan China ; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, 611130 Sichuan China.
出版信息
J Anim Sci Biotechnol. 2015 Aug 8;6(1):34. doi: 10.1186/s40104-015-0032-1. eCollection 2015.
BACKGROUND
This study was carried out to investigate effects of threonine levels on growth, digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp (Ctenopharyngodonidella).
RESULTS
Weight gain, specific growth rate, feed intake and feed efficiency were significantly improved by dietary threonine (P < 0.05). Intestinal activities of trypsin, chymotrypsin, alpha-amylase, lipase, alkaline phosphatase, γ-glutamyl transpeptidase and creatine kinase took the similar trends. Contents of malondialdehyde and protein carbonyl in intestine and hepatopancreas were significantly decreased by dietary optimal threonine supplementation (P < 0.05). Anti-superoxide anion capacity, anti-hydroxyl radical capacity, glutathione content and activities of superoxide dismutase, catalase and glutathione-S-transferase in intestine and hepatopancreas were enhanced by dietary threonine (P < 0.05).
CONCLUSIONS
Dietary threonine could improve growth, enhance digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp. The dietary threonine requirement of sub-adult grass carp (441.9-1,013.4 g) based on weight gain was 11.6 g/kg diet or 41.5 g/kg of dietary protein by quadratic regression analysis.
背景
本研究旨在探讨苏氨酸水平对亚成体草鱼（Ctenopharyngodon idella）生长、消化吸收能力以及肠道和肝胰腺抗氧化状态的影响。
结果
日粮添加苏氨酸显著提高了增重、特定生长率、采食量和饲料效率（P < 0.05）。肠道中胰蛋白酶、糜蛋白酶、α-淀粉酶、脂肪酶、碱性磷酸酶、γ-谷氨酰转肽酶和肌酸激酶的活性呈现相似趋势。日粮添加适宜苏氨酸显著降低了肠道和肝胰腺中丙二醛和蛋白质羰基的含量（P < 0.05）。日粮添加苏氨酸增强了肠道和肝胰腺中的抗超氧阴离子能力、抗羟自由基能力、谷胱甘肽含量以及超氧化物歧化酶、过氧化氢酶和谷胱甘肽-S-转移酶的活性（P < 0.05）。
结论
日粮苏氨酸可改善亚成体草鱼的生长，增强其肠道和肝胰腺的消化吸收能力及抗氧化状态。通过二次回归分析，基于增重的亚成体草鱼（441.9 - 1013.4 g）日粮苏氨酸需求量为11.6 g/kg日粮或41.5 g/kg日粮蛋白质。
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