Jung Keun-Ok, Park Su-Young, Park Kun-Young
Department of Food Science and Nutrition, Pusan National University, Busan, Korea.
Nutrition. 2006 May;22(5):539-45. doi: 10.1016/j.nut.2005.11.007. Epub 2006 Feb 28.
Fermented soy foods, such as doenjang, are important components of the Korean diet. Doenjang has been shown to have greater antimutagenic and anticancer activities than other fermented soybean foods such as chungkukjang (Korean-style natto) and miso and non-fermented soybeans. This study investigated the effects of fermentation time of doenjang on its inhibitory activity against solid tumor formation and lung metastasis.
Effects of methanol extracts from doenjang prepared with long-term fermentation on tumor formation, natural killer cell activity in spleen, and glutathione S-transferase activity in liver were investigated in sarcoma-180 cell-transplanted mice. Inhibitory effects of these samples on lung metastasis of colon 26-M3.1 cells were also evaluated in Balb/c mice. To determine the effect of aging on functionality, doenjang fermented for 3, 6, and 24 mo were evaluated.
Doenjang fermented for 24 mo exhibited a two- to three-fold increase in antitumor effects on sarcoma-180-injected mice and antimetastatic effects in colon 26-M 3.1 cells in mice compared with the 3- or 6-mo fermented doenjang. The 24-mo fermentation was the most effective in preventing cancer by decreasing tumor formation and increasing natural killer cell activity in spleens and glutathione S-transferase activity in livers of mice.
Prolonging the fermentation period when making doenjang increases its antitumor and antimetastatic effects in vivo.
发酵大豆食品，如大酱，是韩国饮食的重要组成部分。已表明大酱比其他发酵大豆食品如清国酱（韩式纳豆）、味噌以及未发酵大豆具有更强的抗诱变和抗癌活性。本研究调查了大酱发酵时间对其抑制实体瘤形成和肺转移活性的影响。
在接种肉瘤-180细胞的小鼠中，研究了长期发酵制备的大酱甲醇提取物对肿瘤形成、脾脏自然杀伤细胞活性以及肝脏谷胱甘肽S-转移酶活性的影响。还在Balb/c小鼠中评估了这些样品对结肠26-M3.1细胞肺转移的抑制作用。为了确定陈化对功能的影响，对发酵3个月、6个月和24个月的大酱进行了评估。
与发酵3个月或6个月的大酱相比，发酵24个月的大酱对注射肉瘤-180的小鼠的抗肿瘤作用以及对小鼠结肠26-M3.1细胞的抗转移作用提高了两到三倍。24个月的发酵在通过减少肿瘤形成以及增加小鼠脾脏自然杀伤细胞活性和肝脏谷胱甘肽S-转移酶活性方面对预防癌症最为有效。
制作大酱时延长发酵期可增强其在体内的抗肿瘤和抗转移作用。
