Dohan David M, Choukroun Joseph, Diss Antoine, Dohan Steve L, Dohan Anthony J J, Mouhyi Jaafar, Gogly Bruno
Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V, Paris, France.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e51-5. doi: 10.1016/j.tripleo.2005.07.010.
Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this third article, we investigate the immune features of this biomaterial. During PRF processing, leucocytes could also secrete cytokines in reaction to the hemostatic and inflammatory phenomena artificially induced in the centrifuged tube. We therefore undertook to quantify 5 significant cell mediators within platelet poor plasma supernatant and PRF clot exudate serum: 3 proinflammatory cytokines (IL-1beta, IL-6, and TNF-alpha), an antiinflammatory cytokine (IL-4), and a key growth promoter of angiogenesis (VEGF). Our data are correlated with that obtained in plasma (nonactivated blood) and in sera (activated blood). These initial analyses revealed that PRF could be an immune regulation node with inflammation retrocontrol abilities. This concept could explain the reduction of postoperative infections when PRF is used as surgical additive.
富血小板纤维蛋白（PRF）属于新一代血小板浓缩物，其加工过程简化，无需对血液进行生化处理。在这第三篇文章中，我们研究了这种生物材料的免疫特性。在PRF制备过程中，白细胞也会因离心管中人为诱导的止血和炎症现象而分泌细胞因子。因此，我们着手对血小板贫乏血浆上清液和PRF凝块渗出液血清中的5种重要细胞介质进行定量：3种促炎细胞因子（IL-1β、IL-6和TNF-α）、1种抗炎细胞因子（IL-4）和1种血管生成关键生长促进因子（VEGF）。我们的数据与在血浆（未激活血液）和血清（激活血液）中获得的数据相关。这些初步分析表明，PRF可能是一个具有炎症反馈控制能力的免疫调节节点。这一概念可以解释当PRF用作手术添加剂时术后感染减少的原因。
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