Dohan David M, Choukroun Joseph, Diss Antoine, Dohan Steve L, Dohan Anthony J J, Mouhyi Jaafar, Gogly Bruno
Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V, Paris, France.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e45-50. doi: 10.1016/j.tripleo.2005.07.009. Epub 2006 Jan 10.
Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this second article, we investigate the platelet-associated features of this biomaterial. During PRF processing by centrifugation, platelets are activated and their massive degranulation implies a very significant cytokine release. Concentrated platelet-rich plasma platelet cytokines have already been quantified in many technologic configurations. To carry out a comparative study, we therefore undertook to quantify PDGF-BB, TGFbeta-1, and IGF-I within PPP (platelet-poor plasma) supernatant and PRF clot exudate serum. These initial analyses revealed that slow fibrin polymerization during PRF processing leads to the intrinsic incorporation of platelet cytokines and glycanic chains in the fibrin meshes. This result would imply that PRF, unlike the other platelet concentrates, would be able to progressively release cytokines during fibrin matrix remodeling; such a mechanism might explain the clinically observed healing properties of PRF.
富血小板纤维蛋白（PRF）属于新一代血小板浓缩物，其加工过程简化，无需对血液进行生化处理。在第二篇文章中，我们研究了这种生物材料与血小板相关的特性。在通过离心进行PRF加工过程中，血小板被激活，其大量脱颗粒意味着会释放非常大量的细胞因子。在许多技术配置中，已对浓缩的富血小板血浆中的血小板细胞因子进行了定量。因此，为了进行比较研究，我们着手对乏血小板血浆（PPP）上清液和PRF凝块渗出血清中的血小板衍生生长因子BB（PDGF-BB）、转化生长因子β-1（TGFbeta-1）和胰岛素样生长因子-I（IGF-I）进行定量。这些初步分析表明，PRF加工过程中纤维蛋白聚合缓慢会导致血小板细胞因子和聚糖链内在地掺入纤维蛋白网中。这一结果意味着，与其他血小板浓缩物不同，PRF能够在纤维蛋白基质重塑过程中逐渐释放细胞因子；这样一种机制可能解释了临床上观察到的PRF的愈合特性。
