Goshima J, Goldberg V M, Caplan A I
Department of Biology, University Hospitals, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106.
Clin Orthop Relat Res. 1991 Aug(269):274-83.
When porous calcium phosphate ceramic is combined with marrow cells and grafted either heterotopically or orthotopically, bone forms inside the pores on the surface of the ceramic beginning at three weeks after implantation. The question remains as to whether the newly formed bone is derived from host or donor cells. To study the origin of bone cells formed in these composite grafts of marrow cells and ceramic, quail marrow cells from long bones were introduced into ceramics and the composites were implanted into subcutaneous pouches of immunologically nonreactive athymic nude mice. The ceramics were recovered at two to 84 days following surgery, fixed, decalcified, embedded, sectioned, and examined for the location of a quail-specific nucleolar marker and the binding of a specific antiserum against quail cells. Our observations indicate that ceramic-associated osteogenesis is a biphasic phenomenon: an early phase, the first three to four weeks after implantation, in which donor cells are largely responsible for the observed osteogenesis, and a second phase, eight to 12 weeks postsurgery, in which host cell actions predominate. During the second stage, the ceramic pores begin to show the formation of marrow of host origin, and the mesenchymal marrow component appears to be osteogenic because the bone formed during this late postgrafting stage contains osteocytes of host and donor origin. The second phase therefore results in chimeric bone composed of quail and mouse. These studies clearly document the donor origin of the initial bone formation and indicate that marrow contains progenitor cells capable of forming de novo bone.
当多孔磷酸钙陶瓷与骨髓细胞结合并异位或原位移植时，植入后三周开始，陶瓷表面的孔隙内会形成骨组织。新形成的骨组织是来源于宿主细胞还是供体细胞，这一问题仍然存在。为了研究在骨髓细胞与陶瓷的这些复合移植物中形成的骨细胞的起源，将来自长骨的鹌鹑骨髓细胞引入陶瓷中，并将复合材料植入免疫无反应的无胸腺裸鼠的皮下袋中。在手术后2至84天回收陶瓷，进行固定、脱钙、包埋、切片，并检查鹌鹑特异性核仁标记物的位置以及针对鹌鹑细胞的特异性抗血清的结合情况。我们的观察结果表明，陶瓷相关的骨生成是一种双相现象：早期阶段，即植入后的前三至四周，在此阶段观察到的骨生成主要由供体细胞负责；第二阶段，即手术后八至十二周，在此阶段宿主细胞的作用占主导地位。在第二阶段，陶瓷孔隙开始显示出宿主来源的骨髓形成，并且间充质骨髓成分似乎具有成骨能力，因为在移植后期形成的骨组织中含有宿主和供体来源的骨细胞。因此，第二阶段会产生由鹌鹑和小鼠组成的嵌合骨。这些研究清楚地证明了初始骨形成的供体来源，并表明骨髓中含有能够从头形成骨的祖细胞。
