Ohgushi H, Okumura M
Department of Orthopedics, Nara Medical University, Japan.
Acta Orthop Scand. 1990 Oct;61(5):431-4. doi: 10.3109/17453679008993556.
Porous hydroxyapatite ceramics, alone and combined with rat marrow cells, were implanted subcutaneously in 22 nude mice. The ceramics alone were invaded by fibrovascular tissue without any bone formation. In contrast, all the ceramics combined with marrow cells had bone formation in the pores 4 to 8 weeks after implantation. The bone formation began on the surface of the ceramic with direct bonding of the bone to the ceramic and proceeded to the center of the pores. The ceramics were also combined with bone marrow cells from 7 humans and implanted in nude mice. In five experiments, bone formation occurred after implantation. In addition, the ceramics were combined with in vitro cultured fibroblastic cells, resulting in bone formation in 2/6 cases. Our results indicate that the osteogenic ability of human marrow cells is sustained by porous hydroxyapatite ceramics.
将多孔羟基磷灰石陶瓷单独以及与大鼠骨髓细胞联合，皮下植入22只裸鼠体内。单独的陶瓷被纤维血管组织侵入，未形成任何骨组织。相比之下，所有与骨髓细胞联合的陶瓷在植入后4至8周时，孔隙内有骨组织形成。骨组织形成始于陶瓷表面，骨与陶瓷直接结合，并向孔隙中心发展。这些陶瓷还与7名人类的骨髓细胞联合，并植入裸鼠体内。在5次实验中，植入后出现了骨组织形成。此外，陶瓷与体外培养的成纤维细胞联合，6例中有2例形成了骨组织。我们的结果表明，多孔羟基磷灰石陶瓷可维持人骨髓细胞的成骨能力。
