Collins D C, Balikian H M, Preedy J R
Endocrinology. 1975 Jun;96(6):1543-51. doi: 10.1210/endo-96-6-1543.
Although hydrolysis of certain estrogen conjugates released into the systemic circulation is now known to occur in the human and in the dog, the site or sites of such hydrolysis remained unidentified. In an attempt to identify such sites, radioactive estrone was infused intravenously into a series of male dogs and serial paired samples of blood were obtained from the right ventricle (RV) and a peripheral artery (A). The samples were analysed for total radioactivity, unconjugated radioactive estrogens, radioactive estrone, estradiol-17beta, estrone sulfate, estrone glucosiduronate and estradiol-17beta glucosiduronate(s). The mean percent extraction across the lungs (see article) was calculated for each radioactive moiety analyzed. There was no significant percent extration of total radioactivity or of estrone sulfate. The mean percent extractions for unconjugated estrogens and for estrone and estradiol-17beta were -24.7, -21 and -34.3 respectively (P less than 0.01), indicating net production by the lungs. The mean percent extractions for estrone glucosiduronate and estradiol-17beta glucosiduronate(s) were +19.8 and +10.0 respectively (P less then 0.01), indicating net uptake of these conjugates by the lungs. Statistical analysis showed that the two processes were related. Our findings support the hypothesis that the lungs are one site, possibly the main site, at which hydrolysis of estrogen glucosiduronates occurs in the dog.
尽管现已知道某些释放到体循环中的雌激素结合物在人和狗体内会发生水解，但这种水解发生的部位仍未明确。为了确定这些部位，将放射性雌酮静脉注射到一系列雄性狗体内，并从右心室（RV）和外周动脉（A）采集连续配对的血样。对这些血样分析总放射性、未结合的放射性雌激素、放射性雌酮、雌二醇-17β、硫酸雌酮、雌酮葡糖醛酸酯和雌二醇-17β葡糖醛酸酯。计算每个分析的放射性部分经肺的平均提取百分比（见文章）。总放射性或硫酸雌酮的提取百分比无显著差异。未结合雌激素、雌酮和雌二醇-17β的平均提取百分比分别为-24.7、-21和-34.3（P小于0.01），表明肺有净生成。雌酮葡糖醛酸酯和雌二醇-17β葡糖醛酸酯的平均提取百分比分别为+19.8和+10.0（P小于0.01），表明肺对这些结合物有净摄取。统计分析表明这两个过程相关。我们的研究结果支持这样的假设，即肺是狗体内雌激素葡糖醛酸酯发生水解的一个部位，可能是主要部位。
