通过荧光原位杂交将编码人细胞色素c氧化酶亚基VIb的基因定位于19号染色体q13.1带。
Assignment of the gene coding for human cytochrome c oxidase subunit VIb to chromosome 19, band q13.1, by fluorescence in situ hybridisation.
作者信息
Taanman J W, van der Veen A Y, Schrage C, de Vries H, Buys C H
机构信息
Laboratory of Physiological Chemistry, University of Groningen, The Netherlands.
出版信息
Hum Genet. 1991 Jul;87(3):325-7. doi: 10.1007/BF00200913.
A cloned, 40 kb, genomic DNA fragment, containing the last exon of the gene for human cytochrome c oxidase subunit VIb and its flanking sequences, was used as a probe to localize the subunit VIb gene on human metaphase chromosomes. The probe was labelled with Bio-11-dUTP and detected by fluorescence. Subsequent R-banding indicated that the cytochrome c oxidase subunit VIb gene is localized in band 19q13.1, extending the evidence that the human nuclear genes of cytochrome c oxidase are not clustered.
一个克隆的40 kb基因组DNA片段，包含人类细胞色素c氧化酶亚基VIb基因的最后一个外显子及其侧翼序列，被用作探针来将亚基VIb基因定位到人类中期染色体上。该探针用生物素-11-dUTP标记并通过荧光检测。随后的R带显示细胞色素c氧化酶亚基VIb基因定位于19q13.1带，进一步证明细胞色素c氧化酶的人类核基因不是成簇的。
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