Taanman J W, Schrage C, Reuvekamp P, Bijl J, Hartog M, de Vries H, Agsteribbe E
Laboratory of Physiological Chemistry, University of Groningen, The Netherlands.
Gene. 1991 Jun 30;102(2):237-44. doi: 10.1016/0378-1119(91)90083-n.
Three pseudogenes for the nuclear-encoded subunit VIb of cytochrome c oxidase (COX) were isolated by screening a human genomic library with cloned human cDNA coding for COX subunit VIb. The nucleotide sequences of the pseudogenes, designated psi COX6b-1, psi COX6b-2 and psi COX6b-3, were determined. Pseudogene psi COX6b-1 bears all the hallmarks of a processed pseudogene and diverged from the parental gene after the divergence of man and cow. Alu repetitive elements were integrated into the structural sequences of the other two pseudogenes. Comparison with the human and bovine cDNA sequences encoding COX subunit VIb suggests that psi COX6b-2 and psi COX6b-3 were formed earlier in evolution than psi COX6b-1. Genomic Southern analysis indicated that a few more pseudogenes for COX subunit VIb are likely to be present in the human genome. Identical nt differences with respect to the human cDNA sequence in the pseudogenes provide some clues on the evolution of the ancestral gene coding for COX subunit VIb.
通过用编码细胞色素c氧化酶（COX）亚基VIb的克隆人cDNA筛选人基因组文库，分离出了COX核编码亚基VIb的三个假基因。确定了这些假基因的核苷酸序列，分别命名为psi COX6b - 1、psi COX6b - 2和psi COX6b - 3。假基因psi COX6b - 1具有加工假基因的所有特征，在人和牛分化之后从亲本基因分化而来。Alu重复元件整合到了另外两个假基因的结构序列中。与编码COX亚基VIb的人和牛cDNA序列比较表明，psi COX6b - 2和psi COX6b - 3在进化中比psi COX6b - 1形成得更早。基因组Southern分析表明，人基因组中可能还存在更多COX亚基VIb的假基因。假基因中相对于人cDNA序列相同的核苷酸差异为编码COX亚基VIb的祖先基因的进化提供了一些线索。
