Lomax M I, Welch M D, Darras B T, Francke U, Grossman L I
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109.
Gene. 1990 Feb 14;86(2):209-16. doi: 10.1016/0378-1119(90)90281-u.
We have isolated a chimpanzee processed pseudogene for subunit IV of cytochrome c oxidase (COX; EC 1.9.3.1) by screening a chimpanzee genomic library in lambda Charon 32 with a bovine liver cDNA encoding COX subunit IV (COX IV), and localized it to a 1.9-kb HindIII fragment. Southern-blot analysis of genomic DNA from five primates showed that DNAs from human, gorilla, and chimpanzee each contained the 1.9-kb pseudogene fragment, whereas orangutan and pigtail macaque monkey DNA did not. This result clearly indicates that the pseudogene arose before the divergence of the chimpanzee and gorilla from the primate lineage. By screening Chinese hamster x human hybrid panels with the human COX4 cDNA, we have mapped COX4 genes to two human chromosomes, 14 and 16. The 1.9-kb HindIII fragment containing the pseudogene, COX4P1, can be assigned to chromosome 14, and by means of rearranged chromosomes in somatic cell hybrids, to 14q21-qter. Similarly, the functional gene, COX4, has been mapped to 16q22-qter.
我们通过用编码细胞色素c氧化酶亚基IV（COX；EC 1.9.3.1）的牛肝cDNA筛选λ噬菌体Charon 32中的黑猩猩基因组文库，分离出了一个黑猩猩加工的细胞色素c氧化酶亚基IV假基因，并将其定位到一个1.9kb的HindIII片段上。对五种灵长类动物基因组DNA的Southern印迹分析表明，人类、大猩猩和黑猩猩的DNA均含有1.9kb的假基因片段，而猩猩和猪尾猕猴的DNA则没有。这一结果清楚地表明，该假基因在黑猩猩和大猩猩从灵长类谱系分化之前就已出现。通过用人COX4 cDNA筛选中国仓鼠×人类杂种细胞系，我们已将COX4基因定位到人类的两条染色体14和16上。含有假基因COX4P1的1.9kb HindIII片段可定位于染色体14，并通过体细胞杂种中的重排染色体定位于14q21-qter。同样，功能基因COX4已被定位到16q22-qter。
