通过聚合酶链反应检测人疱疹病毒6型。

Detection of HHV-6 by the polymerase chain reaction.

作者信息

Collandre H, Aubin J T, Agut H, Béchet J M, Montagnier L

机构信息

Institut Pasteur Unité d'Oncologie Virale, Paris, France.

出版信息

J Virol Methods. 1991 Feb-Mar;31(2-3):171-9. doi: 10.1016/0166-0934(91)90155-s.

DOI:10.1016/0166-0934(91)90155-s

PMID:1650783

Abstract

The polymerase chain reaction (PCR) was used to detect human herpes virus 6 (HHV-6) sequences in tissue culture. A pair of primers was synthesized and used to amplify a conserved region of the genome. Amplified products were detected either by visualization of UV illuminated ethidium bromide stained gel or, by hybridization with a specific radiolabeled oligonucleotide. As little as 5 fg of HHV-6 could be detected in infected cells, making this assay suitable for diagnostic purposes.

摘要

聚合酶链反应（PCR）被用于检测组织培养中的人疱疹病毒6型（HHV - 6）序列。合成了一对引物并用于扩增基因组的一个保守区域。扩增产物通过紫外光照射下溴化乙锭染色凝胶的可视化检测，或者通过与特定放射性标记的寡核苷酸杂交来检测。在受感染细胞中可检测到低至5飞克的HHV - 6，使得该检测方法适用于诊断目的。