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通过Southern印迹法和聚合酶链反应可以区分人类疱疹病毒6型毒株中的几个组。

Several groups among human herpesvirus 6 strains can be distinguished by Southern blotting and polymerase chain reaction.

作者信息

Aubin J T, Collandre H, Candotti D, Ingrand D, Rouzioux C, Burgard M, Richard S, Huraux J M, Agut H

机构信息

Laboratoire de Bactériologie-Virologie, C.E.R.V.I., Hôpital de la Pitié-Salpêtrière, Paris, France.

出版信息

J Clin Microbiol. 1991 Feb;29(2):367-72. doi: 10.1128/jcm.29.2.367-372.1991.

DOI:10.1128/jcm.29.2.367-372.1991
PMID:1848868
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269769/
Abstract

Eight human herpesvirus 6 (HHV-6) strains were studied by Southern blot and polymerase chain reaction. DNA from infected cells was digested by a panel of restriction enzymes and hybridized with cloned BamHI fragments corresponding to about 30% of the HHV-6 strain SIE genome. In parallel, this DNA was amplified by polymerase chain reaction using pairs of primers derived from the strain SIE nucleotide sequence. Subsequently, amplification products were analyzed by hybridization, digestion with restriction endonucleases, and partial nucleotide sequencing. Overall results indicated that all strains were closely related to one another. However, concordant differences in restriction patterns allowed at least two groups to be distinguished, typified by strains SIE and HST, respectively. Differences between the two groups were found to reflect a limited number of punctual changes in nucleotide sequences. These results strengthen the idea of a unique HHV-6 species with genetic polymorphism. In addition, this study provides useful markers for the diagnosis and molecular epidemiology of HHV-6 infections.

摘要

通过Southern印迹法和聚合酶链反应对8株人疱疹病毒6型（HHV-6）菌株进行了研究。用一组限制性内切酶消化感染细胞的DNA，并与对应于HHV-6菌株SIE基因组约30%的克隆BamHI片段杂交。同时，使用源自菌株SIE核苷酸序列的引物对，通过聚合酶链反应扩增该DNA。随后，通过杂交、限制性内切酶消化和部分核苷酸测序对扩增产物进行分析。总体结果表明，所有菌株彼此密切相关。然而，限制性图谱中的一致差异使得至少可以区分出两组，分别以菌株SIE和HST为代表。发现两组之间的差异反映了核苷酸序列中有限数量的点突变。这些结果强化了HHV-6是具有遗传多态性的独特物种这一观点。此外，本研究为HHV-6感染的诊断和分子流行病学提供了有用的标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d202/269769/753cd2d5c8c2/jcm00038-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d202/269769/5b24f4ca16ee/jcm00038-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d202/269769/fec65f70d6c1/jcm00038-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d202/269769/753cd2d5c8c2/jcm00038-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d202/269769/5b24f4ca16ee/jcm00038-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d202/269769/fec65f70d6c1/jcm00038-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d202/269769/753cd2d5c8c2/jcm00038-0154-a.jpg

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本文引用的文献

1
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2
Characteristics of human herpesvirus-6.人类疱疹病毒6型的特征
J Infect Dis. 1988 Jun;157(6):1271-3. doi: 10.1093/infdis/157.6.1271.
3
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J Virol. 2021 Nov 9;95(23):e0126921. doi: 10.1128/JVI.01269-21. Epub 2021 Sep 22.
4
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Virus Evol. 2020 Apr 30;6(1):veaa035. doi: 10.1093/ve/veaa035. eCollection 2020 Jan.
5
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J Virol. 2020 Jun 16;94(13). doi: 10.1128/JVI.00311-20.
6
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J Virol. 2019 Jun 14;93(13). doi: 10.1128/JVI.02321-18. Print 2019 Jul 1.
7
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8
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9
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4
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5
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6
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7
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8
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Lancet. 1988 Mar 26;1(8587):712. doi: 10.1016/s0140-6736(88)91520-6.
10
DNA homology between a novel human herpesvirus (HHV-6) and human cytomegalovirus.一种新型人类疱疹病毒（HHV - 6）与人类巨细胞病毒之间的DNA同源性。
Lancet. 1988;1(8575-6):63-4. doi: 10.1016/s0140-6736(88)91049-5.