Higuchi Ryuichi, Inoue Shinichi, Inagaki Kouji, Sakai Maki, Miyamoto Tomofumi, Komori Tetsuya, Inagaki Masanori, Isobe Ryuichi
Faculty of Pharmaceutical Sciences, Kyushu University, Maidashi, Fukuoka, Japan.
Chem Pharm Bull (Tokyo). 2006 Mar;54(3):287-91. doi: 10.1248/cpb.54.287.
A ganglioside molecular species GP-3 (1) has been obtained from the water-soluble lipid fraction of the chloroform/methanol extract of the starfish Asterina pectinifera. The structure of the ganglioside has been determined on the basis of chemical and spectroscopic evidence. Compound 1 represents new ganglioside molecular species possessing two moles of sialic acids at the inner part of the sugar moiety. Partial hydrolysis by hot water and an enzymatic hydrolysis by means of endoglycoceramidase (EGCase) have proved useful for structure elucidation of the complex oligosaccharide moiety. Moreover, 1 exhibits neuritogenic activity toward the rat pheochromocytoma cell line, PC-12 cells, in the presence of nerve growth factor (NGF).
已从多棘海盘车（Asterina pectinifera）的氯仿/甲醇提取物的水溶性脂质部分中获得了一种神经节苷脂分子物种GP-3 (1)。基于化学和光谱学证据确定了该神经节苷脂的结构。化合物1代表一种新的神经节苷脂分子物种，在糖部分的内部含有两摩尔唾液酸。热水部分水解和通过内切糖神经酰胺酶（EGCase）进行的酶促水解已被证明对复杂寡糖部分的结构解析有用。此外，在神经生长因子（NGF）存在的情况下，1对大鼠嗜铬细胞瘤细胞系PC-12细胞表现出神经突生长活性。
