Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi 10000, Vietnam.
Mar Drugs. 2013 Aug 13;11(8):2917-26. doi: 10.3390/md11082917.
Inflammation is important in biomedical research, because it plays a key role in inflammatory diseases including rheumatoid arthritis and other forms of arthritis, diabetes, heart disease, irritable bowel syndrome, Alzheimer's disease, Parkinson's disease, allergies, asthma, and even cancer. In the present study, we describe the inhibitory effect of crude extracts and steroids isolated from the starfish Astropecten polyacanthus on pro-inflammatory cytokine (Interleukin-12 (IL-12) p40, interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α)) production in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs). Among those tested, compounds 5 and 7 showed potent inhibitory effects on the production of all three pro-inflammatory cytokines with IC50 values ranging from 1.82 ± 0.11 to 7.00 ± 0.16 μM. Potent inhibitory activities were also observed for compound 1 on the production of IL-12 p40 and IL-6 with values of 3.96 ± 0.12 and 4.07 ± 0.13 μM, respectively, and for compounds 3 and 4 on the production of IL-12 p40 with values of 6.55 ± 0.18 and 5.06 ± 0.16 μM, respectively. Moreover, compounds 2 (IC50 = 34.86 ± 0.31 μM) and 6 (IC50 = 79.05 ± 2.05 μM) exhibited moderate inhibitory effects on the production of IL-12 p40, whereas compounds 3 (IC50 = 22.80 ± 0.21 μM) and 4 (IC50 = 16.73 ± 0.25 μM) moderately inhibited the production of TNF-α and IL-6, respectively.
炎症在生物医学研究中很重要，因为它在包括类风湿关节炎和其他形式的关节炎、糖尿病、心脏病、肠易激综合征、阿尔茨海默病、帕金森病、过敏、哮喘甚至癌症在内的炎症性疾病中起着关键作用。在本研究中，我们描述了从星状多棘星虫（Astropecten polyacanthus）中分离得到的粗提物和甾体对脂多糖（LPS）刺激的骨髓来源树突状细胞（BMDC）中促炎细胞因子（白细胞介素-12（IL-12）p40、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α））产生的抑制作用。在所测试的化合物中，化合物 5 和 7 对所有三种促炎细胞因子的产生均表现出强大的抑制作用，IC50 值范围为 1.82±0.11 至 7.00±0.16 μM。化合物 1 对 IL-12 p40 和 IL-6 的产生也表现出强烈的抑制活性，IC50 值分别为 3.96±0.12 和 4.07±0.13 μM，化合物 3 和 4 对 IL-12 p40 的产生的 IC50 值分别为 6.55±0.18 和 5.06±0.16 μM。此外，化合物 2（IC50=34.86±0.31 μM）和 6（IC50=79.05±2.05 μM）对 IL-12 p40 的产生表现出中等抑制作用，而化合物 3（IC50=22.80±0.21 μM）和 4（IC50=16.73±0.25 μM）对 TNF-α 和 IL-6 的产生分别表现出中等抑制作用。
