来自枯草芽孢杆菌的甲基硫代核糖-1-磷酸异构酶的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis.
作者信息
Tamura Haruka, Matsumura Hiroyoshi, Inoue Tsuyoshi, Ashida Hiroki, Saito Yohtaro, Yokota Akiho, Kai Yasushi
机构信息
Department of Materials Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jun 1;61(Pt 6):595-8. doi: 10.1107/S1744309105015757.
Abstract
Methylthioribose-1-phosphate isomerase (MtnA) from Bacillus subtilis, the first enzyme in the downstream section of the methionine-salvage pathway, was crystallized using the sitting-drop vapour-diffusion method. Crystals grew using ammonium sulfate as the precipitant at 293 K. They diffracted to 2.5 A at 100 K using synchrotron radiation and were found to belong to the tetragonal space group P4(1), with unit-cell parameters a = b = 69.2, c = 154.7 A. The asymmetric unit contains two molecules of MtnA, with a VM value of 2.4 A3 Da(-1) and a solvent content of 48%.
摘要
来自枯草芽孢杆菌的甲基硫代核糖-1-磷酸异构酶（MtnA）是甲硫氨酸补救途径下游部分的首个酶，采用坐滴气相扩散法进行了结晶。在293 K下使用硫酸铵作为沉淀剂晶体生长。在100 K下使用同步辐射它们的衍射分辨率达到2.5 Å，并且发现属于四方晶系空间群P4(1)，晶胞参数a = b = 69.2，c = 154.7 Å。不对称单元包含两个MtnA分子，VM值为2.4 Å3 Da(-1)，溶剂含量为48%。
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