环（苯丙氨酸-脯氨酸）调节弧菌属中ompU的表达。
Cyclo(Phe-Pro) modulates the expression of ompU in Vibrio spp.
作者信息
Park Dae-Kyun, Lee Ko-Eun, Baek Chang-Ho, Kim In Hwang, Kwon Ji-Hyoun, Lee Won Koo, Lee Kyu-Ho, Kim Byung-Soo, Choi Sang-Ho, Kim Kun-Soo
机构信息
Department of Life Science, Sogang University, Sinsoo-Dong 1, Mapo-Gu, Seoul 121-742, South Korea.
出版信息
J Bacteriol. 2006 Mar;188(6):2214-21. doi: 10.1128/JB.188.6.2214-2221.2006.
Abstract
Vibrio vulnificus was found to produce a chemical that induced the expression of Vibrio fischeri lux genes. Electron spray ionization-mass spectrometry and 1H nuclear magnetic resonance analyses indicated that the compound was cyclo(L-Phe-L-Pro) (cFP). The compound was produced at a maximal level when cell cultures reached the onset of stationary phase. Sodium dodecyl sulfate-polyacrylamide gel analysis of the total proteins of V. vulnificus indicated that expression of OmpU was enhanced by exogenously added synthetic or purified cFP. A toxR-null mutant failed to express ompU despite the addition of cFP. The related Vibrio spp. V. cholerae, V. parahaemolyticus, and V. harveyi also produced cFP, which induced the expression of their own ompU genes. cFP also enhanced the expression in V. cholerae of the ctx genes, which are known to be regulated by ToxR. Our results suggest that cFP is a signal molecule controlling the expression of genes important for the pathogenicity of Vibrio spp.
摘要
人们发现创伤弧菌能产生一种可诱导费氏弧菌荧光素酶基因表达的化学物质。电喷雾电离质谱分析和1H核磁共振分析表明该化合物为环（L-苯丙氨酸-L-脯氨酸）（cFP）。当细胞培养物进入稳定期开始时，该化合物的产生达到最高水平。对创伤弧菌总蛋白进行的十二烷基硫酸钠-聚丙烯酰胺凝胶分析表明，外源添加的合成或纯化cFP可增强外膜蛋白U（OmpU）的表达。尽管添加了cFP，但一个toxR基因缺失突变体仍无法表达ompU。相关的弧菌属细菌，如霍乱弧菌、副溶血性弧菌和哈维氏弧菌也能产生cFP，它们能诱导自身ompU基因的表达。cFP还增强了霍乱弧菌中已知受ToxR调控的霍乱毒素基因（ctx基因）的表达。我们的研究结果表明，cFP是一种控制对弧菌属致病性至关重要的基因表达的信号分子。
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