Tuffin I Marla, Hector Stanton B, Deane Shelly M, Rawlings Douglas E
Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Appl Environ Microbiol. 2006 Mar;72(3):2247-53. doi: 10.1128/AEM.72.3.2247-2253.2006.
Two sets of arsenic resistance genes were isolated from the highly arsenic-resistant Leptospirillum ferriphilum Fairview strain. One set is located on a transposon, TnLfArs, and is related to the previously identified TnAtcArs from Acidithiobacillus caldus isolated from the same arsenopyrite biooxidation tank as L. ferriphilum. TnLfArs conferred resistance to arsenite and arsenate and was transpositionally active in Escherichia coli. TnLfArs and TnAtcArs were sufficiently different for them not to have been transferred from one type of bacterium to the other in the biooxidation tank. The second set of arsenic resistance genes conferred very low levels of resistance in E. coli and appeared to be poorly expressed in both L. ferriphilum and E. coli.
从高度耐砷的嗜铁钩端螺旋体美景菌株中分离出两组抗砷基因。一组位于转座子TnLfArs上，与先前从与嗜铁钩端螺旋体分离自同一毒砂生物氧化罐的嗜酸性氧化亚铁硫杆菌中鉴定出的TnAtcArs相关。TnLfArs赋予对亚砷酸盐和砷酸盐的抗性，并且在大肠杆菌中具有转座活性。TnLfArs和TnAtcArs差异足够大，以至于它们在生物氧化罐中并非从一种细菌转移至另一种细菌。第二组抗砷基因在大肠杆菌中赋予的抗性水平非常低，并且在嗜铁钩端螺旋体和大肠杆菌中似乎都表达不佳。
