Dave Shailesh R, Gupta Kajal H, Tipre Devayani R
Department of Microbiology, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380 009, India.
Bioresour Technol. 2008 Nov;99(16):7514-20. doi: 10.1016/j.biortech.2008.02.019. Epub 2008 Mar 25.
Four arsenic resistant ferrous oxidizers were isolated from Hutti Gold Mine Ltd. (HGML) samples. Characterization of these isolates was done using conventional microbiological, biochemical and molecular methods. The ferrous oxidation rates with these isolates were 16, 48, 34 and 34 mg L(-1)h(-1) and 15, 47, 34 and 32 mg L(-1)h(-1) in absence and presence of 20 mM of arsenite (As3+) respectively. Except isolate HGM 8, other three isolates showed 2.9-6.3% inhibition due to the presence of 20 mM arsenite. Isolate HGM 8 was able to grow in presence of 14.7 g L(-1) of arsenite, with 25.77 mg L(-1)h(-1) ferrous oxidation rate. All the four isolates were able to oxidize iron and arsenopyrite from 20 g L(-1) and 40 g L(-1) refractory gold ore and 20 g L(-1) refractory gold concentrate. Once the growth was established pH adjustment was not needed inspite of ferrous oxidation, which could be due to concurrent oxidation of pyrite. Isolate HGM 8 showed the final cell count of as high as 1.12 x 10(8) cells mL(-1) in 40 g L(-1) refractory gold ore. The isolates were grouped into one haplotypes by amplified ribosomal DNA restriction analysis (ARDRA). The phylogenetic position of HGM 8 was determined by 16S rDNA sequencing. It was identified as Acidithiobacillus ferrooxidans and strain name was given as SRHGM 1.
从胡蒂金矿有限公司（HGML）的样本中分离出了四株抗砷亚铁氧化菌。使用传统的微生物学、生物化学和分子方法对这些分离菌株进行了表征。在不存在和存在20 mM亚砷酸盐（As3+）的情况下，这些分离菌株的亚铁氧化速率分别为16、48、34和34 mg L(-1)h(-1)以及15、47、34和32 mg L(-1)h(-1)。除了分离菌株HGM 8外，其他三株分离菌株由于存在20 mM亚砷酸盐而表现出2.9 - 6.3%的抑制率。分离菌株HGM 8能够在14.7 g L(-1)亚砷酸盐存在的情况下生长，亚铁氧化速率为25.77 mg L(-1)h(-1)。所有这四株分离菌株都能够氧化来自20 g L(-1)和40 g L(-1)难处理金矿以及20 g L(-1)难处理金精矿中的铁和毒砂。一旦生长确立，尽管存在亚铁氧化，但无需进行pH调节，这可能是由于黄铁矿的同时氧化。在40 g L(-1)难处理金矿中，分离菌株HGM 8的最终细胞计数高达1.12 x 10(8) 个细胞 mL(-1)。通过扩增核糖体DNA限制性分析（ARDRA）将这些分离菌株归为一个单倍型。通过16S rDNA测序确定了HGM 8的系统发育位置。它被鉴定为嗜酸氧化亚铁硫杆菌，并命名为SRHGM 1菌株。
