Semret Makeda, Turenne Christine Y, de Haas Petra, Collins Desmond M, Behr Marcel A
McGill University Health Centre, Montreal, Quebec, Canada.
J Clin Microbiol. 2006 Mar;44(3):881-7. doi: 10.1128/JCM.44.3.881-887.2006.
The Mycobacterium avium species consists of a group of organisms that are genetically related but phenotypically diverse, with certain variants presenting clear differences in terms of their host association and disease manifestations. The ability to distinguish between these subtypes is of relevance for accurate diagnosis and for control programs. Using a comparative genomics approach, we have uncovered large sequence polymorphisms that are, respectively, absent from bird-type M. avium isolates and from cattle types and sheep types of M. avium subsp. paratuberculosis. By evaluating the distribution of these genomic polymorphisms across a panel of strains, we were able to assign unique genomic signatures to these host-associated variants. We propose a simple PCR-based strategy based on these polymorphisms that can rapidly type M. avium isolates into these subgroups.
鸟分枝杆菌属由一组在遗传上相关但表型多样的生物体组成，某些变体在宿主关联和疾病表现方面存在明显差异。区分这些亚型的能力对于准确诊断和控制计划具有重要意义。通过比较基因组学方法，我们发现了大片段序列多态性，这些多态性分别不存在于鸟型鸟分枝杆菌分离株以及副结核分枝杆菌亚种的牛型和羊型中。通过评估这些基因组多态性在一组菌株中的分布，我们能够为这些宿主相关变体赋予独特的基因组特征。我们基于这些多态性提出了一种简单的基于PCR的策略，该策略可以快速将鸟分枝杆菌分离株分型为这些亚组。
