Stepanović Srdjan, Hauschild Tomasz, Dakić Ivana, Al-Doori Zainab, Svabić-Vlahović Milena, Ranin Lazar, Morrison Donald
Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, Dr Subotića 1, 11000 Belgrade, Serbia.
J Clin Microbiol. 2006 Mar;44(3):934-7. doi: 10.1128/JCM.44.3.934-937.2006.
In this paper we report on an experimental evaluation of phenotypic and molecular methods as means for the detection of oxacillin resistance in members of the Staphylococcus sciuri group. A total of 109 S. sciuri group member isolates (92 S. sciuri isolates, 9 S. lentus isolates, and 8 S. vitulinus isolates) were tested by the disk diffusion method, the agar dilution method, the oxacillin salt-agar screening method, slide latex agglutination for PBP 2a, and PCR assay for mecA as the reference method. The mecA gene was detected in 29 S. sciuri isolates, and the true-positive and true-negative results of the other tests were defined on the basis of the presence or the absence of the mecA gene. For the different methods evaluated, the sensitivities and specificities were as follows: for the disk diffusion test with a 1-microg oxacillin disk, 100% and 55.9%, respectively; for the disk diffusion test with a 30-mug cefoxitin disk, 93.5% and 100%, respectively; for the agar dilution method, 100% and 50%, respectively; for the oxacillin salt-agar screen test (with 6 microg of oxacillin per ml and 4% NaCl) 100% and 100%, respectively; and for the slide latex agglutination test for PBP 2a, 100% and 100%, respectively. The disk diffusion test with various beta-lactam antibiotics was performed to evaluate their use for the prediction of oxacillin resistance. The results indicate that meropenem, cefazolin, cefamandole, cefuroxime, cefotetan, cefoperazone, cefotaxime, ceftriaxone, moxalactam, cefaclor, and cefprozil may be used as surrogate markers of oxacillin resistance, although further studies of their use for the detection of oxacillin resistance are required.
在本文中，我们报告了对表型和分子方法作为检测松鼠葡萄球菌属成员中苯唑西林耐药性手段的实验评估。通过纸片扩散法、琼脂稀释法、苯唑西林盐琼脂筛选法、PBP 2a的玻片乳胶凝集试验以及作为参考方法的mecA基因PCR检测，对总共109株松鼠葡萄球菌属成员分离株（92株松鼠葡萄球菌分离株、9株缓慢葡萄球菌分离株和8株维氏葡萄球菌分离株）进行了检测。在29株松鼠葡萄球菌分离株中检测到了mecA基因，其他检测的真阳性和真阴性结果根据mecA基因的有无来定义。对于所评估的不同方法，其敏感性和特异性如下：对于使用1μg苯唑西林纸片的纸片扩散试验，分别为100%和55.9%；对于使用30μg头孢西丁纸片的纸片扩散试验，分别为93.5%和100%；对于琼脂稀释法，分别为100%和50%；对于苯唑西林盐琼脂筛选试验（每毫升含6μg苯唑西林和4%氯化钠），分别为100%和100%；对于PBP 2a的玻片乳胶凝集试验，分别为100%和100%。进行了使用各种β-内酰胺抗生素的纸片扩散试验，以评估它们用于预测苯唑西林耐药性的情况。结果表明，美罗培南、头孢唑林、头孢孟多、头孢呋辛、头孢替坦、头孢哌酮、头孢噻肟、头孢曲松、拉氧头孢、头孢克洛和头孢丙烯可作为苯唑西林耐药性的替代标志物，尽管需要进一步研究它们在检测苯唑西林耐药性方面的应用。
