Capitanio N, Capitanio G, De Nitto E, Villani G, Papa S
Institute of Medical Biochemistry and Chemistry, Faculty of Medicine, University of Bari, Italy.
FEBS Lett. 1991 Aug 19;288(1-2):179-82. doi: 10.1016/0014-5793(91)81029-8.
The H+/e- stoichiometry of protonmotive cytochrome c oxidase, isolated from bovine heart mitochondria and reconstituted in liposomes, has been determined by making use of direct spectrophotometric measurements of the initial rates of e- flow and H+ translocation. It is shown that the ----H+/e- ratio for redox-linked proton ejection by the oxidase varies from around 0 to a maximum of 1 as a function of the rate of overall electron flow in the complex.
从牛心线粒体中分离并重组到脂质体中的质子动力细胞色素c氧化酶的H⁺/e⁻化学计量比，已通过直接分光光度法测量电子流和H⁺转运的初始速率来确定。结果表明，氧化酶氧化还原偶联质子排出的H⁺/e⁻比值随复合物中总电子流速率的变化而变化，从约0到最大值1。
