Papa S, Capitanio N, Capitanio G, De Nitto E, Minuto M
Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
FEBS Lett. 1991 Aug 19;288(1-2):183-6. doi: 10.1016/0014-5793(91)81030-c.
A study is presented of the ----H+/e- stoichiometry for H+ pumping by the cytochrome chain in isolated rat liver mitochondria under level-flow and steady-state conditions. It is shown that the ----H+/e- stoichiometry for the cytochrome chain varies under the influence of the flow rate and transmembrane delta microH+. The rate-dependence is shown to be associated with cytochrome c oxidase, whose ----H+/e- ratio varies from 0 to 1, whilst the ----H+/e- ratio for the span covered by cytochrome c reductase is invariably 2.
本文呈现了一项关于在平流和稳态条件下，分离的大鼠肝脏线粒体中细胞色素链进行质子泵浦时的H⁺/e⁻化学计量比的研究。结果表明，细胞色素链的H⁺/e⁻化学计量比在流速和跨膜ΔμH⁺的影响下会发生变化。速率依赖性被证明与细胞色素c氧化酶有关，其H⁺/e⁻比值在0到1之间变化，而细胞色素c还原酶覆盖范围内的H⁺/e⁻比值始终为2。
