[染料木黄酮对涎腺腺样囊性癌细胞系SACC-83细胞周期蛋白表达的影响]
[Effects of genistein on the expressions of cell cycle proteins in salivary adenoid cystic carcinoma cell line SACC-83].
作者信息
Ma Jie, Wang Jie, Zhong Ming, Wang Zhao-yuan
机构信息
Department of Oral Medicine, Second Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.
出版信息
Shanghai Kou Qiang Yi Xue. 2006 Feb;15(1):69-72.
PURPOSE
To investigate the molecular mechanism of cell cycle arrest induced by tyrosine protein kinase inhibitor, genistein, in human salivary adenoid cystic carcinoma cell line SACC-83.
METHODS
SACC-83 cells cultured in vitro were treated with genistein, the expressions of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins were detected with Western blotting, and the results were quantitatively analyzed by FluorChem V2.0 software, statistical analysis was performed with analysis of variance using SPSS11.5 software.
RESULTS
With the increase of concentration of genistein and the elongation of time, the expression of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins was significantly decreased. Treated with 220 micromol/L of genistein for 3 days, the expression level of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins was 58%, 64%, 46% and 43% of the control group, respectively (P<0.01).
CONCLUSION
The cell cycle G2/M arrest induced by genistein in human salivary adenoid cystic carcinoma cell line SACC-83 may be associated with the downregulations of CyclinB1, Cdk1, CyclinD1 and Cdk4 protein expressions.
目的
探讨酪氨酸蛋白激酶抑制剂染料木黄酮诱导人涎腺腺样囊性癌细胞系SACC - 83细胞周期阻滞的分子机制。
方法
体外培养的SACC - 83细胞用染料木黄酮处理，采用蛋白质印迹法检测细胞周期蛋白B1（CyclinB1）、细胞周期蛋白依赖性激酶1（Cdk1）、细胞周期蛋白D1（CyclinD1）和细胞周期蛋白依赖性激酶4（Cdk4）蛋白的表达，并用FluorChem V2.0软件进行定量分析，采用SPSS11.5软件进行方差分析。
结果
随着染料木黄酮浓度的增加和作用时间的延长，CyclinB1、Cdk1、CyclinD1和Cdk4蛋白的表达显著降低。用220μmol/L染料木黄酮处理3天，CyclinB1、Cdk1、CyclinD1和Cdk4蛋白的表达水平分别为对照组的58%、64%、46%和43%（P<0.01）。
结论
染料木黄酮诱导人涎腺腺样囊性癌细胞系SACC - 83细胞周期G2/M期阻滞可能与CyclinB1、Cdk1、CyclinD1和Cdk4蛋白表达下调有关。
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