Chow Yung-Chiong, Yang Stone, Huang Chun-Jen, Tzen Chin-Yuan, Huang Pei-Lin, Su Yu-Hsien, Wang Paulus S
Department of Urology, Mackay Memorial Hospital, Medicine, Nursing, and Management College, Tapei, Taiwan.
Urology. 2006 Mar;67(3):636-41. doi: 10.1016/j.urology.2005.10.003.
To evaluate the hypothesis that intravesical instillation of epinephrine would attenuate bladder hemorrhage in a rat model of cyclophosphamide (CYP)-induced hemorrhagic cystitis.
Female Sprague-Dawley rats were divided into seven treatment groups: positive control (CYP, 150 mg/kg), negative control (epinephrine, 10 microg/mL), intravesical instillation of normal saline (vehicle) and epinephrine (2.5, 5, and 10 microg/mL), and intraperitoneal administration of mesna (50 mg/kg). Rats were killed on days 1, 2, and 3, and the urinary bladders were removed, weighed, and evaluated by gross and histologic analysis. Vesical vascular permeability was determined by wet bladder weight and Evan's blue dye absorbance.
Cyclophosphamide administration induced severe hemorrhagic cystitis with marked edema, hemorrhage, and inflammation. All three epinephrine-treated groups had marked attenuation of hemorrhagic cystitis compared with the positive and negative control and mesna-treated groups. Epinephrine was also associated with significant inhibition of tissue edema, indicating decreased vesical vascular permeability.
In this rat model of CYP-induced hemorrhagic cystitis, intravesical instillation of epinephrine inhibited edema, hemorrhage, and inflammation.
评估在环磷酰胺（CYP）诱导的出血性膀胱炎大鼠模型中，膀胱内灌注肾上腺素是否会减轻膀胱出血。
将雌性Sprague-Dawley大鼠分为七个治疗组：阳性对照组（CYP，150mg/kg）、阴性对照组（肾上腺素，10μg/mL）、膀胱内灌注生理盐水（赋形剂）和肾上腺素（2.5、5和10μg/mL），以及腹腔注射美司钠（50mg/kg）。在第1、2和3天处死大鼠，取出膀胱，称重，并通过大体和组织学分析进行评估。通过膀胱湿重和伊文思蓝染料吸光度测定膀胱血管通透性。
给予环磷酰胺可诱发严重的出血性膀胱炎，并伴有明显的水肿、出血和炎症。与阳性对照组、阴性对照组和美司钠治疗组相比，所有三个肾上腺素治疗组的出血性膀胱炎均有明显减轻。肾上腺素还与组织水肿的显著抑制相关，表明膀胱血管通透性降低。
在这个CYP诱导的出血性膀胱炎大鼠模型中，膀胱内灌注肾上腺素可抑制水肿、出血和炎症。
