Diehl Michael R, Zhang Kechun, Lee Heun Jin, Tirrell David A
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Science. 2006 Mar 10;311(5766):1468-71. doi: 10.1126/science.1122125.
A biosynthetic approach was developed to control and probe cooperativity in multiunit biomotor assemblies by linking molecular motors to artificial protein scaffolds. This approach provides precise control over spatial and elastic coupling between motors. Cooperative interactions between monomeric kinesin-1 motors attached to protein scaffolds enhance hydrolysis activity and microtubule gliding velocity. However, these interactions are not influenced by changes in the elastic properties of the scaffold, distinguishing multimotor transport from that powered by unorganized monomeric motors. These results highlight the role of supramolecular architecture in determining mechanisms of collective transport.
通过将分子马达与人工蛋白质支架相连，开发了一种生物合成方法来控制和探测多单元生物马达组件中的协同作用。这种方法可对马达之间的空间和弹性耦合进行精确控制。附着在蛋白质支架上的单体驱动蛋白-1马达之间的协同相互作用增强了水解活性和微管滑动速度。然而，这些相互作用不受支架弹性特性变化的影响，这使得多马达运输与由无组织的单体马达驱动的运输区分开来。这些结果突出了超分子结构在决定集体运输机制中的作用。
