Tim Taylor Department of Chemical Engineering, Kansas State University, Manhattan, KS 66506, USA.
Int J Mol Sci. 2020 May 19;21(10):3584. doi: 10.3390/ijms21103584.
Coiled-coils, the bundles of intertwined helical protein motifs, have drawn much attention as versatile molecular toolkits. Because of programmable interaction specificity and affinity as well as well-established sequence-to-structure relationships, coiled-coils have been used as subunits that self-assemble various molecular complexes in a range of fields. In this review, I describe recent advances in the field of protein nanotechnology, with a focus on programming assembly of protein nanostructures using coiled-coil modules. Modular design approaches to converting the helical motifs into self-assembling building blocks are described, followed by a discussion on the molecular basis and principles underlying the modular designs. This review also provides a summary of recently developed nanostructures with a variety of structural features, which are in categories of unbounded nanostructures, discrete nanoparticles, and well-defined origami nanostructures. Challenges existing in current design strategies, as well as desired improvements for controls over material properties and functionalities for applications, are also provided.
螺旋线圈，即螺旋蛋白基序交织成的束状结构，作为多功能的分子工具包引起了广泛关注。由于可编程的相互作用特异性和亲和力以及成熟的序列-结构关系，螺旋线圈已被用作亚基，在多个领域中自组装各种分子复合物。在这篇综述中，我描述了蛋白质纳米技术领域的最新进展，重点是使用螺旋线圈模块来编程组装蛋白质纳米结构。描述了将螺旋基序转化为自组装构建块的模块化设计方法，然后讨论了模块化设计的分子基础和原理。这篇综述还总结了最近开发的具有各种结构特征的纳米结构，这些纳米结构分为无边界纳米结构、离散纳米颗粒和明确定义的折纸纳米结构。还提供了当前设计策略中存在的挑战，以及对材料性能和功能进行控制以应用于所需的改进。
