Jeansson S, Elwing H, Nilsson L A
J Clin Microbiol. 1979 Mar;9(3):317-22. doi: 10.1128/jcm.9.3.317-322.1979.
Thin-layer immunoassay (TIA) is a simple serological technique suitable for analysis of large numbers of samples. In this study, TIA was evaluated for determination of antibodies to herpes simplex virus. Herpes simplex virus antigen used in TIA was purified from material released from virus-infected cells. The results obtained by TIA were compared with those obtained by neutralization and complement fixation tests. TIA was found to be as sensitive as the neutralization test for demonstration of herpes simplex virus antibodies. No false-negative or -positive reactions were observed. In primary herpes simplex virus-1 infections, an antibody response was demonstrated by TIA, whereas antibodies could not be demonstrated in patients with primary herpes simplex virus-2 infections.
薄层免疫测定法(TIA)是一种适用于大量样本分析的简单血清学技术。在本研究中,对TIA用于检测单纯疱疹病毒抗体的情况进行了评估。TIA中使用的单纯疱疹病毒抗原是从病毒感染细胞释放的物质中纯化得到的。将TIA获得的结果与通过中和试验和补体结合试验获得的结果进行了比较。发现TIA在检测单纯疱疹病毒抗体方面与中和试验一样敏感。未观察到假阴性或假阳性反应。在原发性1型单纯疱疹病毒感染中,TIA显示出抗体反应,而在原发性2型单纯疱疹病毒感染患者中未检测到抗体。