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[一种通过对单一血清稀释液进行免疫酶分析来测定单纯疱疹病毒抗体滴度的简单快速方法]

[A simple and rapid method of determining the titer of herpes simplex virus antibodies in a study by immunoenzyme analysis of a single serum dilution].

作者信息

Kibardin V M, Ebralidze L K, Zvonarev A Iu

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1989 Jan(1):61-3.

PMID:2540601
Abstract

The result obtained in the study of the possibility of using the method for the determination of the titer of antibodies to herpes simplex virus by EIA techniques in a single dilution of the serum under test are presented. This method is based on the determination of the optical density of the serum titer (rcut) in different groups of sera with the use of the assay system, permitting the evaluation of the positive results obtained in the determination of their final dilution. The results obtained with the use of this method showed that error was 50% for high-titer sera, 60% for medium-titer sera and 30% for low-titer sera.

摘要

本文展示了一项研究结果,该研究探讨了采用酶免疫分析技术在单一稀释度的待测血清中测定单纯疱疹病毒抗体滴度方法的可能性。此方法基于利用检测系统测定不同血清组中血清滴度(rcut)的光密度,从而能够评估在最终稀释度测定中获得的阳性结果。使用该方法获得的结果表明,高滴度血清的误差为50%,中滴度血清的误差为60%,低滴度血清的误差为30%。

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