Dittmar Michael S, Vatankhah Bijan, Fehm Nando P, Schuierer Gerhard, Bogdahn Ulrich, Horn Markus, Schlachetzki Felix
Department of Anesthesiology, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
J Neurosci Methods. 2006 Sep 30;156(1-2):50-4. doi: 10.1016/j.jneumeth.2006.02.003.
Middle cerebral artery occlusion (MCAO) in Fischer-344 rats results in a small variance of infarct size. However, complications are frequent especially in aged Fisher-344 rats undergoing endovascular suture occlusion of the middle cerebral artery. Analyzing our experiences with 165 Wistar, 13 Sprague-Dawley and 10 F-344 rats, we compared the incidence of impossible thread advancement and subarachnoid hemorrhage, respectively. Magnetic resonance angiography (MRA) was applied to study the course of the internal carotid artery (ICA) in Fischer and Wistar rats. Finally, we performed a structured review of the literature from 1991 to 2005 evaluating reports on Fischer rats subjected to intraluminal filament MCAO. Complications like fruitless filament advancement or subarachnoid hemorrhage were found to be significantly more frequent in Fischer rats than in other strains. MRA revealed significantly more pronounced kinking of the ICA in F-344 than in Wistar rats. In seven publications available on filament MCAO in F-344 rats, complication rates of 50-100% were reported, corroborating our data. Surgical difficulties accompanied by high complication rates due to their cerebrovascular anatomy make Fischer rats unsuitable for filament MCAO. If the use of Fischer rats for studies on focal cerebral ischemia is indicated, other ischemia models than intraluminal suture occlusion should be chosen.
在Fischer-344大鼠中，大脑中动脉闭塞（MCAO）导致梗死灶大小的差异较小。然而，并发症很常见，尤其是在接受大脑中动脉血管内缝合闭塞术的老年Fisher-344大鼠中。分析我们对165只Wistar大鼠、13只Sprague-Dawley大鼠和10只F-344大鼠的实验经验，我们分别比较了缝线推进困难和蛛网膜下腔出血的发生率。应用磁共振血管造影（MRA）研究了Fischer大鼠和Wistar大鼠的颈内动脉（ICA）走行。最后，我们对1991年至2005年的文献进行了结构化回顾，评估了关于Fischer大鼠接受腔内细丝MCAO的报告。发现Fischer大鼠中诸如细丝推进无果或蛛网膜下腔出血等并发症明显比其他品系更常见。MRA显示F-344大鼠的ICA迂曲比Wistar大鼠更明显。在七篇关于F-344大鼠细丝MCAO的可用出版物中，报告的并发症发生率为50%-100%，证实了我们的数据。由于其脑血管解剖结构，手术困难并伴有高并发症发生率使得Fischer大鼠不适合进行细丝MCAO。如果表明要使用Fischer大鼠进行局灶性脑缺血研究，应选择腔内缝合闭塞以外的其他缺血模型。
