Dittmar Michael S, Vatankhah Bijan, Fehm Nando P, Retzl Gerald, Schuierer Gerhard, Bogdahn Ulrich, Schlachetzki Felix, Horn Markus
Department of Anesthesiology, University of Regensburg, 93042 Regensburg, Germany.
Exp Neurol. 2005 Oct;195(2):372-8. doi: 10.1016/j.expneurol.2005.05.013.
In the intraluminal suture model of middle cerebral artery occlusion (MCAO) in the rat, lesions of the masticator muscles associated with impaired functional outcome occur. We evaluated the role of external carotid artery (ECA) transection. We assessed whether isolated interruption of an arterial or a venous connection to the ECA territory was sufficient to induce masticatory hypoperfusion and lesions. We also evaluated a direct access to the common carotid artery (CCA) with subsequent vascular closure with regard to its feasibility, frequency of masticatory lesions, complications, and cerebral ischemia. Cerebral and masticatory lesions and perfusion deficits were assessed by in vivo magnetic resonance imaging (MRI). Vessel patency was evaluated using computerized tomography angiography and histology. An interruption of arterial blood flow led to masticatory hypoperfusion. Masticatory lesions occurred in 6% of the rats. Access to and closure of the CCA were feasible in all animals, leading to moderate or severe vessel stenosis in 20%, and intraarterial thrombosis in 25% of the rats. Reproducible cerebral infarctions were obtained in all animals. In 24% of the rats, hyperintense MRI signal changes were observed in the ipsilateral temporal muscle. Thus, the induction of masticatory hypoperfusion and lesions by arterial transection supports the role of the ECA in this context. Direct access to the CCA with subsequent vessel closure led to stenosis in most animals. Preservation of ECA continuity was not suitable to fully prevent masticatory lesions.
在大鼠大脑中动脉闭塞（MCAO）的管腔内缝合模型中，出现了与功能预后受损相关的咀嚼肌损伤。我们评估了颈外动脉（ECA）横断的作用。我们评估了单独中断与ECA区域的动脉或静脉连接是否足以诱发咀嚼肌灌注不足和损伤。我们还评估了直接进入颈总动脉（CCA）并随后进行血管闭合的可行性、咀嚼肌损伤的发生率、并发症以及脑缺血情况。通过体内磁共振成像（MRI）评估脑和咀嚼肌损伤以及灌注不足。使用计算机断层血管造影和组织学评估血管通畅情况。动脉血流中断导致咀嚼肌灌注不足。6%的大鼠出现了咀嚼肌损伤。在所有动物中，进入和闭合CCA都是可行的，20%的大鼠出现中度或重度血管狭窄，25%的大鼠出现动脉内血栓形成。所有动物均获得了可重复的脑梗死。24%的大鼠在同侧颞肌中观察到高强度MRI信号变化。因此，动脉横断诱发咀嚼肌灌注不足和损伤支持了ECA在这种情况下的作用。直接进入CCA并随后进行血管闭合导致大多数动物出现狭窄。保留ECA的连续性并不足以完全预防咀嚼肌损伤。
