从乌桕树下采集的土壤中通过质谱法鉴定出一种佛波酯12-O-十六烷酰佛波醇-13-乙酸酯，一种爱泼斯坦-巴尔病毒激活物质。

Mass spectrometric identification of a phorbol diester 12-O-hexadecanoylphorbol-13-acetate, an Epstein-Barr virus-activating substance, in the soil collected from under Sapium sebiferum.

作者信息

Takeda N, Ohigashi H, Hirai N, Koshimizu K, Suzuki M, Tatematsu A, Osato T, Mizuno F

机构信息

Faculty of Pharmacy, Meijo University, Nagoya, Japan.

出版信息

Cancer Lett. 1991 Aug;59(2):153-8. doi: 10.1016/0304-3835(91)90180-p.

DOI:10.1016/0304-3835(91)90180-p

PMID:1653107

Abstract

Soil-extracts collected from the ground from under several Euphorbiaceae plants have been known to possess Epstein-Barr virus (EBV)-activating substances which are thought to be one of the environmental co-factors causing nasopharyngeal carcinoma (NPC) in southern part of China and Burkitt's lymphoma (BL) in tropical Africa. Then, a model experiment aimed at chemical characterization of such active substances was carried out using a soil-extract around Sapium sebiferum, a Japanese representative Euphorbiaceae plant. Chromatographic separation guided by the EBV early antigen (EA) inducing activity gave a highly active fraction. Application of this fraction to desorption chemical ionization mass spectrometry identified a major active substance to be 12-O-hexadecanoylphorbol-13-acetate (HPA), which originally occurs in this plant. The method in this model experiment is suggested to be applicable to other samples from the endemic areas of NPC and BL.

摘要

从几种大戟科植物下方地面采集的土壤提取物已知含有爱泼斯坦-巴尔病毒（EBV）激活物质，这些物质被认为是中国南部导致鼻咽癌（NPC）以及热带非洲导致伯基特淋巴瘤（BL）的环境协同因素之一。然后，以日本代表性大戟科植物乌桕周围的土壤提取物为材料，开展了一项旨在对这类活性物质进行化学表征的模型实验。以EBV早期抗原（EA）诱导活性为导向的色谱分离得到了一个高活性组分。将该组分应用于解吸化学电离质谱分析，确定一种主要活性物质为12-O-十六酰佛波醇-13-乙酸酯（HPA），它最初存在于这种植物中。该模型实验中的方法建议可应用于来自NPC和BL流行地区的其他样本。