回旋酶抑制剂会增加大肠杆菌中质粒pBR322的纽结DNA种类的含量。
Gyrase inhibitors increase the content of knotted DNA species of plasmid pBR322 in Escherichia coli.
作者信息
Ishii S, Murakami T, Shishido K
机构信息
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
J Bacteriol. 1991 Sep;173(17):5551-3. doi: 10.1128/jb.173.17.5551-5553.1991.
Abstract
Treatment of Escherichia coli cells harboring pBR322 with the DNA gyrase inhibitors oxolinic acid and coumermycin A1 led to an increase in the content of knotted pBR322 molecules. This phenomenon was attributed to inhibition of gyrase-catalyzed unknotting of the plasmid DNA knotted by transcription.
摘要
用DNA回旋酶抑制剂恶喹酸和香豆霉素A1处理携带pBR322的大肠杆菌细胞，导致打结的pBR322分子含量增加。这种现象归因于回旋酶催化的由转录造成的质粒DNA解结受到抑制。
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