DNA 回旋酶 B 亚基的拮抗作用可使大肠杆菌中的质粒 pBR322 和 pMG110 消除。
Antagonism of the B subunit of DNA gyrase eliminates plasmids pBR322 and pMG110 from Escherichia coli.
作者信息
Wolfson J S, Hooper D C, Swartz M N, McHugh G L
出版信息
J Bacteriol. 1982 Oct;152(1):338-44. doi: 10.1128/jb.152.1.338-344.1982.
Abstract
The constructed plasmid pBR322 and the native plasmid pMG110 were eliminated (cured) from growing Escherichia coli cells by the antagonism of the B subunit of the bacterial enzyme DNA gyrase. The antagonism may be by the growth of cells (i) at semipermissive temperatures in a bacterial mutant containing a thermolabile gyrase B subunit or (ii) at semipermissive concentrations of coumermycin A1, an antibiotic that specifically inhibits the B subunit of DNA gyrase. The kinetics of plasmid elimination indicate that plasmid loss occurs too rapidly to be explained solely by the faster growth of that plasmid-free bacteria and, therefore, represents interference with plasmid maintenance.
摘要
通过细菌酶DNA促旋酶B亚基的拮抗作用，从生长的大肠杆菌细胞中消除（治愈）构建的质粒pBR322和天然质粒pMG110。这种拮抗作用可能是通过以下方式实现的：（i）在含有热不稳定促旋酶B亚基的细菌突变体中，于半允许温度下培养细胞；（ii）在香豆霉素A1（一种特异性抑制DNA促旋酶B亚基的抗生素）的半允许浓度下培养细胞。质粒消除的动力学表明，质粒丢失发生得太快，无法仅用无质粒细菌的更快生长来解释，因此，这代表了对质粒维持的干扰。
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本文引用的文献
1
THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI.吖啶染料对大肠杆菌交配型因子的影响
Proc Natl Acad Sci U S A. 1960 Jan;46(1):57-64. doi: 10.1073/pnas.46.1.57.
2
Episome-mediated transfer of drug resistance in Enterobacteriaceae. II. Elimination of resistance factors with acridine dyes.附加体介导的肠杆菌科细菌耐药性转移。II. 用吖啶染料消除耐药因子。
J Bacteriol. 1961 May;81(5):679-83. doi: 10.1128/jb.81.5.679-683.1961.
3
Acriflavine as an effective agent for eliminating F-factor in Escherichia coli K-12.吖啶黄作为消除大肠杆菌K-12中F因子的有效试剂。
Nature. 1957 Sep 28;180(4587):655-6. doi: 10.1038/180655a0.
4
The maintenance of Plasmid-containing organisms in populations of Escherichia coli.含质粒的大肠杆菌群体的维持
J Gen Microbiol. 1981 Mar;123(1):129-41. doi: 10.1099/00221287-123-1-129.
5
Maintenance of some ColE1-type plasmids in chemostat culture.在恒化器培养中某些ColE1型质粒的维持
Mol Gen Genet. 1980;180(3):579-84. doi: 10.1007/BF00268063.
6
Curing of Escherichia coli K12 plasmids by coumermycin.香豆霉素消除大肠杆菌K12质粒
Mol Gen Genet. 1980 Apr;178(1):233-5. doi: 10.1007/BF00267235.
7
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J Bacteriol. 1981 Dec;148(3):769-81. doi: 10.1128/jb.148.3.769-781.1981.
8
DNA topoisomerases.DNA拓扑异构酶
Annu Rev Biochem. 1981;50:879-910. doi: 10.1146/annurev.bi.50.070181.004311.
9
DNA supercoiling: another level for regulating gene expression.DNA超螺旋：基因表达调控的另一个层面。
Cell. 1981 Jun;24(3):599-600. doi: 10.1016/0092-8674(81)90085-4.
10
Initiation of DNA replication in Bacillus subtilis. V. Role of DNA gyrase and superhelical structure in initiation.枯草芽孢杆菌中DNA复制的起始。V. DNA促旋酶和超螺旋结构在起始过程中的作用。
Mol Gen Genet. 1981;181(3):332-7. doi: 10.1007/BF00425607.
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