Tsikas D, Fauler J, Brunner G, Frölich J C
Department of Clinical Pharmacology, Hannover Medical School, Germany.
J Chromatogr. 1991 Jun 7;545(2):375-8. doi: 10.1016/s0021-9673(01)88729-x.
The cysteinyl leukotrienes (LTs) C4, D4 and E4 are among the most potent lipid mediators of anaphylaxis and inflammation. A capillary isotachophoretic method is described for the determination of these cysteinyl LTs. The method is based on anionic separation and detection using UV (254 nm) and conductivity detectors. The total analysis time is of the order of 30 min. The limit of detection of the method was determined to be 0.5 nmol of LTE4. Despite of similar chemical structures, all three cysteinyl LTs can be determined simultaneously.
半胱氨酰白三烯（LTs）C4、D4和E4是过敏反应和炎症中最有效的脂质介质。本文描述了一种用于测定这些半胱氨酰白三烯的毛细管等速电泳方法。该方法基于阴离子分离，并使用紫外（254 nm）和电导检测器进行检测。总分析时间约为30分钟。该方法的检测限确定为0.5 nmol的LTE4。尽管这三种半胱氨酰白三烯的化学结构相似，但仍可同时进行测定。
