Lecce J G, Leary H L, Clarke D A, Batema R P
Department of Animal Science and Microbiology, North Carolina State University, Raleigh 27695.
J Clin Microbiol. 1991 Jul;29(7):1382-6. doi: 10.1128/jcm.29.7.1382-1386.1991.
Rotavirus, a double-stranded RNA virus, has been implicated as a diarrhea-provoking agent in a variety of animal species. Several previous reports have shown that immunization with a single serotype may result in increased in vitro neutralization titers against serotypes not represented in the immunogen. This study was undertaken to determine whether antibody from cows immunized against simian rotavirus strain SA-11 (which is alien to pigs) could protect neonatal piglets from infection with a North Carolina isolate of porcine rotavirus. Accordingly, cows were immunized with SA-11 and an immunoglobulin G (IgG)-rich fraction was isolated from their colostrum. An IgG-rich fraction was similarly isolated from colostrum of nonimmunized cows. At equal concentrations, IgG from SA-11-immunized cows had two- to fourfold higher neutralization titers to seven of eight test strains of rotavirus, including SA-11 (serotype 3); human rotavirus serotypes 1, 3, and 4; North Carolina porcine rotavirus (serotype undetermined); Ohio State porcine rotavirus (serotype 5); and bovine rotavirus (serotype 6). The IgG-rich fractions were fed as dietary supplements to agammaglobulinemic piglets infected with the North Carolina porcine rotavirus. IgG from the SA-11-immunized cows was about eightfold more effective in protecting piglets than was IgG from nonimmunized cows.
轮状病毒是一种双链RNA病毒，被认为是多种动物腹泻的致病因子。此前有几份报告表明，用单一血清型进行免疫可能会导致体外中和抗体效价升高，对免疫原中未包含的血清型产生作用。本研究旨在确定用针对猿猴轮状病毒SA-11株（对猪来说是异种病毒）免疫的奶牛产生的抗体，是否能保护新生仔猪免受北卡罗来纳州猪轮状病毒分离株的感染。因此，用SA-11对奶牛进行免疫，并从它们的初乳中分离出富含免疫球蛋白G（IgG）的部分。同样从未免疫奶牛的初乳中分离出富含IgG的部分。在相同浓度下，来自用SA-11免疫的奶牛的IgG对8种轮状病毒测试株中的7种，包括SA-11（血清型3）；人轮状病毒血清型1、3和4；北卡罗来纳州猪轮状病毒（血清型未确定）；俄亥俄州猪轮状病毒（血清型5）；以及牛轮状病毒（血清型6）的中和效价高两到四倍。将富含IgG的部分作为膳食补充剂喂给感染了北卡罗来纳州猪轮状病毒的无丙种球蛋白血症仔猪。来自用SA-11免疫的奶牛的IgG在保护仔猪方面的效果比来自未免疫奶牛的IgG高约八倍。
