Wyatt R G, Greenberg H B, James W D, Pittman A L, Kalica A R, Flores J, Chanock R M, Kapikian A Z
Infect Immun. 1982 Jul;37(1):110-5. doi: 10.1128/iai.37.1.110-115.1982.
Twenty different human rotavirus reassortants were characterized serologically by a plaque reduction assay as belonging to one of three distinct serotypes. Fourteen were similar if not identical to our prototype Wa strain; two were like the prototype DS-1 strain, and four belonged to a third serotype for which a prototype has not yet been selected. Hyperimmune sera raised against the three serotypes were required to distinguish among them, since postinfection sera had lower titers and were more cross-reactive than hyperimmune sera. These results confirmed the ability of a qualitative cytopathic neutralization test to predict correctly the Wa or DS-1 serotype. A strain of rhesus rotavirus (MMU 18006) was identified as belonging to the newly defined third serotype. Finally, an attempt was made to correlate previously published serotype analysis by neutralization of fluorescent cell-forming units with the results determined by the plaque reduction neutralization assay.
通过蚀斑减少试验对20种不同的人轮状病毒重配株进行血清学鉴定，结果表明它们分属于三种不同血清型中的一种。其中14株即便不完全相同也与我们的原型株Wa相似；2株类似于原型株DS-1；4株属于尚未选定原型的第三种血清型。由于感染后血清的效价较低且比超免疫血清的交叉反应性更强，因此需要用针对这三种血清型产生的超免疫血清来区分它们。这些结果证实了定性细胞病变中和试验能够正确预测Wa或DS-1血清型。一株恒河猴轮状病毒（MMU 18006）被鉴定为属于新定义的第三种血清型。最后，尝试将先前发表的通过中和荧光细胞形成单位进行的血清型分析结果与蚀斑减少中和试验所确定的结果进行关联。
